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小鼠 anti-人 kappa Light Chain Antibody

FACS, WB, IHC (p), IHC (fro) Monoclonal KLC709 IgG1 kappa unconjugated
产品编号 ABIN3026394
发货至: 中国
  • 抗原 See all kappa Light Chain products
    kappa Light Chain
    适用
    • 34
    • 11
    宿主
    • 17
    • 11
    • 9
    • 4
    • 3
    • 1
    小鼠
    克隆类型
    • 26
    • 19
    单克隆
    应用范围
    • 29
    • 26
    • 18
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    • 14
    • 10
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    Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    产品特性
    This antibody is specific to the kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. In general, the ratio of Kappa to Lambda is 3:1. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
    纯化方法
    Protein G affinity chromatography
    免疫原
    Recombinant human Kappa light chain was used as the immunogen for this anti-Kappa light chain antibody.
    克隆位点
    KLC709
    亚型
    IgG1 kappa
  • 应用备注
    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the anti-Kappa light chain antibody to be titered up or down for optimal performance.

    1. Staining of FFPE tissues requires boiling sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 0.5-1 μg/mL,FACS: 0.5-1 μg/million cells,IHC (Frozen, FFPE): 0.5-1.0 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    限制
    仅限研究用
  • 浓度
    0.2 mg/mL
    缓冲液
    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store the anti-Kappa light chain antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原
    kappa Light Chain
    Abstract
    kappa Light Chain 产品
    背景
    This antibody is specific to the kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. In general, the ratio of Kappa to Lambda is 3:1. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
    基因ID
    3514
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