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TGFA 抗体 (C-Term)

TGFA 适用: 人, 大鼠, 小鼠, Cow WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal MF9 unconjugated
产品编号 ABIN3026161
发货至: 中国
  • 抗原 See all TGFA 抗体
    TGFA (Transforming Growth Factor, alpha (TGFA))
    抗原表位
    • 22
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    C-Term
    适用
    • 105
    • 50
    • 29
    • 16
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    • 1
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    人, 大鼠, 小鼠, Cow
    宿主
    • 67
    • 38
    • 3
    • 2
    • 1
    小鼠
    克隆类型
    • 64
    • 46
    • 1
    单克隆
    标记
    • 56
    • 9
    • 3
    • 3
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    • 3
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    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
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    • 1
    • 1
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    • 1
    This TGFA antibody is un-conjugated
    应用范围
    • 53
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    • 9
    • 5
    • 3
    • 3
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    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    纯化方法
    Protein G affinity chromatography
    免疫原
    A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF alpha was used as the immunogen for this antibody.
    克隆位点
    MF9
    亚型
    IgG1 kappa
    Top Product
    Discover our top product TGFA Primary Antibody
  • 应用备注
    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TGF alpha antibody to be titered up or down for optimal performance.

    1. Staining of FFPE tissues requires boiling sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.\. Western blot: 1-2 μg/mL, FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 min at RT (1)

    限制
    仅限研究用
  • 浓度
    0.2 mg/mL
    缓冲液
    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store the TGF alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原
    TGFA (Transforming Growth Factor, alpha (TGFA))
    别名
    TGF alpha (TGFA 产品)
    别名
    TFGA antibody, LOC100009482 antibody, TGF alpha antibody, TGFA antibody, wa-1 antibody, wa1 antibody, RATTGFAA antibody, TGFAA antibody, NV15606 antibody, si:ch211-10e8.7 antibody, transforming growth factor alpha antibody, transforming growth factor alpha L homeolog antibody, transforming growth factor-alpha antibody, transforming growth factor, alpha antibody, TGFA antibody, TGF-A antibody, Tgfa antibody, tgfa.L antibody, LOC100121300 antibody, LOC100009150 antibody, tgfa antibody
    背景
    TGF alpha is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. Staining with the antibody is completely blocked by the peptide used for raising this antibody.
    基因ID
    7039
    途径
    NF-kappaB Signaling, RTK signaling, EGFR Signaling Pathway
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