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Myc Tag 抗体 (C-Term) (HRP)

适用: 人 WB 宿主: 小鼠 Monoclonal 9E10 HRP
产品编号 ABIN302064
发货至: 中国
  • 抗原 See all Myc Tag products
    Myc Tag
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    C-Term
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    小鼠
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    单克隆
    标记
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    This Myc Tag antibody is conjugated to HRP
    应用范围
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    Western Blotting (WB)
    特异性
    The antibody 9E10 can be used to detect the c-Myc tag.
    交叉反应 (详细)
    Human, Recognizes fusion proteins in all species
    纯化方法
    Purified antibody is conjugated with activated horseradish peroxidase (HRP) of high specific enzyme activity under optimum conditions and unconjugated antibody and free HRP are removed by size-exclusion chromatography.
    免疫原
    Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-Myc.
    克隆位点
    9E10
    亚型
    IgG1
  • 应用备注
    Western blotting: Recommended dilution: 1-2 μg/mL, positive control: c-Myc tagged protein.
    说明

    The purified antibody is conjugated with Horseradish Peroxidase (HRP) of high specific activity and RZ=3.

    限制
    仅限研究用
  • 浓度
    1 mg/mL
    缓冲液
    Stabilizing phosphate buffered saline (PBS), pH 7.4, 0.01 % thimerosal
    储存液
    Thimerosal (Merthiolate)
    注意事项
    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Do not freeze.
    Avoid prolonged exposure to light.
    储存条件
    4 °C
    储存方法
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Jelínková, Šafaříková, Vondálová Blanářová, Skender, Hofmanová, Sova, Moyer, Kozubík, Kolář, Ehrmann, Hyršlová Vaculová: "Platinum(IV) complex LA-12 exerts higher ability than cisplatin to enhance TRAIL-induced cancer cell apoptosis via stimulation of mitochondrial pathway." in: Biochemical pharmacology, Vol. 92, Issue 3, pp. 415-24, (2014) (PubMed).

    Veracini, Simon, Richard, Schraven, Horejsi, Roche, Benistant: "The Csk-binding protein PAG regulates PDGF-induced Src mitogenic signaling via GM1." in: The Journal of cell biology, Vol. 182, Issue 3, pp. 603-14, (2008) (PubMed).

    Wang, Campoli, Ko, Luo, Ferrone: "Enhancement of scFv fragment reactivity with target antigens in binding assays following mixing with anti-tag monoclonal antibodies." in: Journal of immunological methods, Vol. 294, Issue 1-2, pp. 23-35, (2004) (PubMed).

    Fujiwara, Poikonen, Aleman, Valtavaara, Saksela, Mayer: "A single-chain antibody/epitope system for functional analysis of protein-protein interactions." in: Biochemistry, Vol. 41, Issue 42, pp. 12729-38, (2002) (PubMed).

    Baggio, Burgstaller, Hale, Putney, Lane, Lipovsek, Wright, Roberts, Liu, Szostak, Wagner: "Identification of epitope-like consensus motifs using mRNA display." in: Journal of molecular recognition : JMR, Vol. 15, Issue 3, pp. 126-34, (2002) (PubMed).

    Cermák, Símová, Pintzas, Horejsí, Andera: "Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules." in: The Journal of biological chemistry, Vol. 277, Issue 10, pp. 7955-61, (2002) (PubMed).

    Hilpert, Hansen, Wessner, Küttner, Welfle, Seifert, Höhne: "Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose." in: Protein engineering, Vol. 14, Issue 10, pp. 803-6, (2001) (PubMed).

    Siegel, Brandner, Hess: "Cross-reactivity of the monoclonal antibody 9E10 with murine c-MYC." in: International journal of oncology, Vol. 13, Issue 6, pp. 1259-62, (1999) (PubMed).

  • 抗原
    Myc Tag
    别名
    c-Myc tag (Myc Tag 产品)
    物质类
    Tag
    背景
    MYC proto-oncogene,The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase (JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is also essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells.,bHLH, MRTL, MYCC
    基因ID
    4609
    UniProt
    P01106
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