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Iba1 抗体 (C-Term)

Name: Iba1 抗体 (C-Term)
Brand: antibodies-online
SKU: ABIN2857032
Availability: OutOfStock
Rating: 5 (1 reviews)

IBA1 适用: 人 WB, IHC, IHC (p), ICC, IF, FACS, IHC (fro), IHC (ff) 宿主: 兔 Polyclonal unconjugated

Immunofluorescence validation image for anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (C-Term) antibody (ABIN2857032)

IHC-P Image Iba1 antibody detects Iba1 protein on mouse fore brain by immunohistochemical analysis.

IHC

IHC-P Image Iba1 antibody detects Iba1 protein on rat hind brain by immunohistochemical analysis.

IHC

WB Image Iba1 antibody detects Iba1 protein by western blot analysis.

IHC-P Image Iba1 detects Iba1 protein at cytosol on mouse brain tissue by immunohistochemical analysis.

IHC

IHC-P Image Iba1 antibody detects Iba1 protein at cytoplasm on human colon carcinoma stroma by immunohistochemical analysis.

IHC

WB Image Sample (30 ug of whole cell lysate) A: Molt-4 , 15% SDS PAGE AIF1 antibody antibody diluted at 1:1000

IHC-P Image Immunohistochemical analysis of paraffin-embedded human hepatoma, using Iba1, antibody at 1:500 dilution.

IHC

ASI administration deactivated microglial activation and reduced inflammatory responses in spinal cords of EAE mice.

Neuropathology in the central nervous system of Fuca1-deficient mice.

18 images

(13 references)

(1 validation)

产品编号 ABIN2857032

发货至: 中国

Datasheet as PDF

Key Features
- High quality Iba1 Primary Antibody for the detection of IBA1.
- Reliable product with high standard validation data
抗原 See all Iba1 (IBA1) 抗体

Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))
抗原表位
16

16

15

10

9

8

7

7

3

1

1

1

1

1

1

1
C-Term
适用
88

55

48

2

1
人
宿主
76

32

6

1
兔
Compatible Secondaries
克隆类型
78

37
多克隆
标记
55

18

13

7

3

2

2

2

2

2

2

2

1

1

1

1

1
This Iba1 antibody is un-conjugated
应用范围
79

46

43

21

20

14

14

12

11

7

4

4

3

3

2

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Free Floating) (IHC (ff))

交叉反应

人, 小鼠, 大鼠

产品特性

Rabbit Polyclonal antibody to Iba1 (allograft inflammatory factor 1)
Iba1 antibody

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Iba1. The exact sequence is proprietary.

亚型

IgG

Product Specific Information

What can the Iba1 Antibody ABIN2857032 be used for?

This polyclonal Iba1 Antibody detects Iba1. The Iba1 Antibody has been validated for various applications and can be used for the detection of Iba1 and derivatives by Western Blotting, Immunohistochemistry, Immunohistochemistry (Paraffin-embedded Sections), Immunocytochemistry, Immunofluorescence, Flow Cytometry, Immunohistochemistry (Frozen Sections), Immunohistochemistry (Free Floating).

What validation data is available for this Iba1 Antibody?

The Primary Antibody is referenced in 13 publications and is characterized by a proven, very high reliability. It has currently 17 product images that show its performance in a variety of applications. The product is currently available in 100 μL quantity. Iba1 Antibody for the detection of Iba1 and derivatives.

What is the function of Iba1?

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008].
anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 51-147) antibody
IBA1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) antibody
IBA1 适用: 人 WB, IHC, IP, IHC (p), ICC, IF, FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 1-147) antibody
IBA1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 1-147) antibody
IBA1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C5 unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (Internal Region) antibody
Verified IBA1 适用: 人 IHC, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (C-Term) antibody
Verified IBA1 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 1-147) antibody
IBA1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) antibody
IBA1 适用: 小鼠 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) antibody
IBA1 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 35-102) antibody
IBA1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. FACS: 1:50-1:200. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: mouse liver , rat liver
Validation: Orthogonal

限制

仅限研究用
生效 #101924 (Immunofluorescence)

by

Centre d'Immunologie de Marseille-Luminy

No.

#101924

日期

2017.10.19

抗原

AIF1

Lot Number

39476

Method validated

Immunofluorescence

Positive Control

PFA fixed tissue section brain of CX3CR1 GFP mouse. Staining with Iba1 (Wako, 019-19741) and Donkey anti-rabbit Fab’2 AlexaFluor 647

Negative Control

Notes

Passed. Immunostaining of mouse brain samples with ABIN2857032 shows specifically the expected staining pattern.

Validation Images

Colocalization (C) of the signals in PFA fixed tissue sections of CX3CR1 GFP mouse brain for the immunostaining of with ABIN2857032 (A) and GFP (B).
Full Methods
Primary Antibody

ABIN2857032

Secondary Antibody

donkey Fab’2 anti-rabbit A647 conjugated antibody (Jackson Immunoresearch, 711-606-152, lot 128806)

Full Protocol

Harvest Brain from 13 weeks old mice in 1x Dulbecco’s phosphate buffered saline (DPBS) (Gibco Life Technologies, 14200-067).
Fix mouse brain in antigen fix (Diapath, P0014) for 2h at RT.
Wash tissue in 0.1M pH7.4 phosphate buffer at for 1h at 4°C.
Dehydrate tissue in 30% sucrose solution ON at 4°C.
Snap freeze tissue in Tissue Freezing Medium (ElectroMicroscopy Science, 72592-C) at -80°C.
Cut blocks into 20µm sections using a cryostat (Leica, CM3050 S).
Transfer sections to a slide.
Create a hydrophobic barrier on the slide around sections with Dako pen (Dako, S2002, lot 00081640).
Place slide in a humidified chamber and rehydrate sections in 0.1M TrisHCl pH7.4 for 10min at RT.
Gently remove buffer by tapping slide.
Permeabilize tissue in 0.1M TrisHCl pH7.4 containing 2% Triton X-100 (Sigma, batch 015K0039) and 0.5% BSA, for 20min at RT.
Incubate sections with primary AIF1 (c-term) (antibodies-online, ABIN2857032, Lot 39476) diluted 1:500 in 0.1M TrisHCl pH7.4 containing 2% Triton X-100 (Sigma, batch 015K0039) and 0.5% BSA for 5h at RT. Incubate the negative contrls sections with diluted rabbit serum.
Wash slides 1x 5min in 0.1M TrisHCl pH7.4.
Incubate sections with secondary donkey Fab’2 anti-rabbit A647 conjugated antibody (Jackson Immunoresearch, 711-606-152, lot 128806) diluted 1:500 in 0.1M TrisHCl pH7.4 containing 2% Triton X-100 and 0.5% BSA ON at RT.
Wash slides 1x 5min in 0.1M TrisHCl pH7.4.
Add approximately 10μl of Slowfade® Gold antifade reagent (Life technologies, 536937, lot 1226836) for each section and mount cover slip.
Image acquisition on a LSM 880 (Zeiss), 20x magnification, 1000 resolution.

Experimental Notes

Microglial cells in the brain of the CX3CR1 GFP mouse expressing GFP are known to express Aif-1. Immunostaining of the PFA fixed tissue sections with ABIN2857032 showed the expected co-localization of the GFP and Aif-1 signals.
状态

Liquid

浓度

0.06 mg/mL

缓冲液

1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Kaehler, Seitter, Sandbichler, Tschugg, Obermair, Stefanova, Koschak: "Assessment of the Retina of Plp-α-Syn Mice as a Model for Studying Synuclein-Dependent Diseases." in: Investigative ophthalmology & visual science, Vol. 61, Issue 6, pp. 12, (2020) (PubMed).

Wahle, Sofranko, Dekkers, Miller, Heusinkveld, Albrecht, Cassee, Schins: "Evaluation of neurological effects of cerium dioxide nanoparticles doped with different amounts of zirconium following inhalation exposure in mouse models of Alzheimer's and vascular disease." in: Neurochemistry international, Vol. 138, pp. 104755, (2020) (PubMed).

Li, Zhang, Yang, Blevins, Norris, Zhao, Huang: "C-terminal binding proteins 1 and 2 in traumatic brain injury-induced inflammation and their inhibition as an approach for anti-inflammatory treatment." in: International journal of biological sciences, Vol. 16, Issue 7, pp. 1107-1120, (2020) (PubMed).

Mondor, Baratin, Lagueyrie, Saro, Henri, Gentek, Suerinck, Kastenmuller, Jiang, Bajénoff: "Lymphatic Endothelial Cells Are Essential Components of the Subcapsular Sinus Macrophage Niche." in: Immunity, Vol. 50, Issue 6, pp. 1453-1466.e4, (2019) (PubMed).

Olechnowicz, Weivoda, Lwin, Leung, Gooding, Nador, Javaid, Ramasamy, Rao, Edwards, Edwards: "Multiple myeloma increases nerve growth factor and other pain-related markers through interactions with the bone microenvironment." in: Scientific reports, Vol. 9, Issue 1, pp. 14189, (2019) (PubMed).

Wahl, Solon-Biet, Wang, Wali, Pulpitel, Clark, Raubenheimer, Senior, Sinclair, Cooney, de Cabo, Cogger, Simpson, Le Couteur: "Comparing the Effects of Low-Protein and High-Carbohydrate Diets and Caloric Restriction on Brain Aging in Mice." in: Cell reports, Vol. 25, Issue 8, pp. 2234-2243.e6, (2019) (PubMed).

Huang, Lin, Chen, Chen, Chen, Hsu, Wu: "NADPH oxidases as potential pharmacological targets against increased seizure susceptibility after systemic inflammation." in: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 140, (2019) (PubMed).

Götzl, Brendel, Werner, Parhizkar, Sebastian Monasor, Kleinberger, Colombo, Deussing, Wagner, Winkelmann, Diehl-Schmid, Levin, Fellerer, Reifschneider, Bultmann, Bartenstein, Rominger, Tahirovic et al.: "Opposite microglial activation stages upon loss of PGRN or TREM2 result in reduced cerebral glucose metabolism. ..." in: EMBO molecular medicine, Vol. 11, Issue 6, (2019) (PubMed).

Lin, Hsuan, Lin, Kuo, Lin, Su, Niu, Chang, Lin: "Human Umbilical Cord Mesenchymal Stem Cells Preserve Adult Newborn Neurons and Reduce Neurological Injury after Cerebral Ischemia by Reducing the Number of Hypertrophic Microglia/Macrophages." in: Cell transplantation, Vol. 26, Issue 11, pp. 1798-1810, (2018) (PubMed).

Zha, Liu, Zhu, Liu, Lu, Xu, Yu, Liu: "A scFv antibody targeting common oligomeric epitope has potential for treating several amyloidoses." in: Scientific reports, Vol. 6, pp. 36631, (2018) (PubMed).

Zera, Zastre: "Thiamine deficiency activates hypoxia inducible factor-1α to facilitate pro-apoptotic responses in mouse primary astrocytes." in: PLoS ONE, Vol. 12, Issue 10, pp. e0186707, (2017) (PubMed).

Liu, Shi, Huang, Peterson, Wu, Lan, Zhang, He, Woods, Du, Wu, Wang: "Astragaloside IV inhibits microglia activation via glucocorticoid receptor mediated signaling pathway." in: Scientific reports, Vol. 6, pp. 19137, (2016) (PubMed).

Wolf, Damme, Stroobants, DHooge, Beck, Hermans-Borgmeyer, Lüllmann-Rauch, Dierks, Lübke: "A mouse model for fucosidosis recapitulates storage pathology and neurological features of the milder form of the human disease." in: Disease models & mechanisms, Vol. 9, Issue 9, pp. 1015-28, (2016) (PubMed).
抗原

Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

别名

allograft inflammatory factor 1 (IBA1 产品)

别名

AIF-1 antibody, IBA1 antibody, IRT-1 antibody, IRT1 antibody, AI607846 antibody, D17H6S50E antibody, G1 antibody, Iba1 antibody, Bart1 antibody, iba1 antibody, mrf-1 antibody, fa04b11 antibody, wu:fa04b11 antibody, AIF antibody, AIF1 antibody, aif1 antibody, allograft inflammatory factor 1 antibody, AIF1 antibody, Aif1 antibody, aif1 antibody

背景

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene.

分子量

17 kDa

基因ID

199

UniProt

P55008

途径

Smooth Muscle Cell Migration