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HA-Tag 抗体

适用: 人 WB 宿主: 小鼠 Monoclonal 12CA5 unconjugated
产品编号 ABIN2843186
发货至: 中国
  • 抗原 See all HA-Tag products
    HA-Tag
    适用
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    宿主
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    小鼠
    克隆类型
    • 85
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    单克隆
    标记
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    This HA-Tag antibody is un-conjugated
    应用范围
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    Western Blotting (WB)
    序列
    YPYDVPDYA
    纯化方法
    This antibody is purified through a protein G column, followed by dialysis against PBS.
    免疫原
    KLH conjugated synthetic peptide encoding HA tag (YPYDVPDYA) was used as antigen.
    克隆位点
    12CA5
    亚型
    IgG2b
  • 应用备注
    WB: 1:8000
    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid freeze-thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
    有效期
    6 months
  • Milewska, Kindler, Vkovski, Zeglen, Ochman, Thiel, Rajfur, Pyrc: "APOBEC3-mediated restriction of RNA virus replication." in: Scientific reports, Vol. 8, Issue 1, pp. 5960, (2019) (PubMed).

    Li, Guo, Feng, Zhang, Wang, Jing, Yan, Jing, Liu, Ma, Diao: "Sp1 transcription factor promotes TMEPAI gene expression and contributes to cell proliferation." in: Cell proliferation, Vol. 49, Issue 6, pp. 710-719, (2017) (PubMed).

    Tham, Joshi, Moukhles: "Aquaporin-4 Cell-Surface Expression and Turnover Are Regulated by Dystroglycan, Dynamin, and the Extracellular Matrix in Astrocytes." in: PLoS ONE, Vol. 11, Issue 10, pp. e0165439, (2017) (PubMed).

    Schmidt, Goebeler, Martin: "Methods to Investigate the Role of Toll-Like Receptors in Allergic Contact Dermatitis." in: Methods in molecular biology (Clifton, N.J.), Vol. 1390, pp. 319-40, (2016) (PubMed).

    Mertz, Tan, Pagala, Bai, Chen, Li, Cho, Shaw, Wang, Peng: "Sequential Elution Interactome Analysis of the Mind Bomb 1 Ubiquitin Ligase Reveals a Novel Role in Dendritic Spine Outgrowth." in: Molecular & cellular proteomics : MCP, Vol. 14, Issue 7, pp. 1898-910, (2015) (PubMed).

    Mizutani, Inoue, Omori, Ito, Tamiya-Koizumi, Takagi, Kojima, Nakamura, Iwaki, Nakatochi, Suzuki, Nozawa, Murate: "Increased acid ceramidase expression depends on upregulation of androgen-dependent deubiquitinases, USP2, in a human prostate cancer cell line, LNCaP." in: Journal of biochemistry, (2015) (PubMed).

    Drechsler, Tan, Liakopoulos: "Yeast GSK-3 kinase regulates astral microtubule function through phosphorylation of the microtubule-stabilizing kinesin Kip2." in: Journal of cell science, Vol. 128, Issue 21, pp. 3910-21, (2015) (PubMed).

    Li, An, Li, Ma, Feng, Zhang, Guo: "DELLA Proteins Interact with FLC to Repress the Flowering Transition." in: Journal of integrative plant biology, (2015) (PubMed).

    Koo, Yue, Gal, Khuri, Sun: "Maintaining glycogen synthase kinase-3 activity is critical for mTOR kinase inhibitors to inhibit cancer cell growth." in: Cancer research, Vol. 74, Issue 9, pp. 2555-68, (2014) (PubMed).

    Bruntz, Taylor, Lindsley, Brown: "Phospholipase D2 mediates survival signaling through direct regulation of Akt in glioblastoma cells." in: The Journal of biological chemistry, Vol. 289, Issue 2, pp. 600-16, (2014) (PubMed).

    Li, Ren, Yue, Chen, Khuri, Sun: "The novel Akt inhibitor API-1 induces c-FLIP degradation and synergizes with TRAIL to augment apoptosis independent of Akt inhibition." in: Cancer prevention research (Philadelphia, Pa.), Vol. 5, Issue 4, pp. 612-20, (2012) (PubMed).

    Sun, Ni: "HYPOSENSITIVE TO LIGHT, an alpha/beta fold protein, acts downstream of ELONGATED HYPOCOTYL 5 to regulate seedling de-etiolation." in: Molecular plant, Vol. 4, Issue 1, pp. 116-26, (2011) (PubMed).

    Czymai, Viemann, Sticht, Molema, Goebeler, Schmidt: "FOXO3 modulates endothelial gene expression and function by classical and alternative mechanisms." in: The Journal of biological chemistry, Vol. 285, Issue 14, pp. 10163-78, (2010) (PubMed).

    Herrera, Silva, Garvin: "Angiotensin II stimulates thick ascending limb superoxide production via protein kinase C(?)-dependent NADPH oxidase activation." in: The Journal of biological chemistry, Vol. 285, Issue 28, pp. 21323-8, (2010) (PubMed).

    Kauh, Fan, Xia, Yue, Yang, Khuri, Sun: "c-FLIP degradation mediates sensitization of pancreatic cancer cells to TRAIL-induced apoptosis by the histone deacetylase inhibitor LBH589." in: PLoS ONE, Vol. 5, Issue 4, pp. e10376, (2010) (PubMed).

    Nishikawa, Nakashima, Hayashi, Fukunaga, Kato, Kodama, Takahashi, Calame, Takayanagi: "Blimp1-mediated repression of negative regulators is required for osteoclast differentiation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 7, pp. 3117-22, (2010) (PubMed).

    Flozak, Lam, Russell, Jain, Peled, Sheppard, Beri, Mutlu, Budinger, Gottardi: "Beta-catenin/T-cell factor signaling is activated during lung injury and promotes the survival and migration of alveolar epithelial cells." in: The Journal of biological chemistry, Vol. 285, Issue 5, pp. 3157-67, (2010) (PubMed).

    Satow, Shitashige, Jigami, Honda, Ono, Hirohashi, Yamada: "Traf2- and Nck-interacting kinase is essential for canonical Wnt signaling in Xenopus axis formation." in: The Journal of biological chemistry, Vol. 285, Issue 34, pp. 26289-94, (2010) (PubMed).

    Guo, Thomas, Collins, Timmermans: "Direct repression of KNOX loci by the ASYMMETRIC LEAVES1 complex of Arabidopsis." in: The Plant cell, Vol. 20, Issue 1, pp. 48-58, (2008) (PubMed).

    Chen, Liu, Yue, Schönthal, Khuri, Sun et al.: "CCAAT/enhancer binding protein homologous protein-dependent death receptor 5 induction and ubiquitin/proteasome-mediated cellular FLICE-inhibitory protein down-regulation contribute to enhancement of ..." in: Molecular pharmacology, Vol. 72, Issue 5, pp. 1269-79, (2007) (PubMed).

  • 抗原
    HA-Tag
    Abstract
    HA-Tag 产品
    物质类
    Tag
    背景
    Epitope tags consisting of short sequences recognized by well-characterizated monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. Abgent's anti-HA monoclonal antibody (Clone 12CA5) provides a simple solution to detect the expression of an HA-tagged protein in cells.
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