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Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (N-Term) 抗体

ABIN2782428 产品详细信息, 供应商: Log in to see
抗原
  • p40phox
  • AI451400
  • NCF
  • P40PHOX
  • SH3PXD4
  • p40phox protein
  • neutrophil cytosolic factor 4
  • neutrophil cytosolic factor 4, 40kDa
  • p40phox
  • LOC100136241
  • Ncf4
  • NCF4
抗原表位
N-Term
19
9
9
4
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
适用
牛, 犬, 豚鼠, 马, 人, 小鼠, 猪, 兔, 大鼠
117
57
43
6
6
4
3
3
3
2
宿主

90
20
11
克隆类型
多克隆
标记
非结合性
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
应用范围
Western Blotting (WB)
74
41
33
26
23
5
4
3
3
2
2
1
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免疫原 The immunogen is a synthetic peptide directed towards the N terminal region of human NCF4
Blocking Peptide Blocking peptide for this product available: ABIN980522
序列 SKYLIYRRYR QFHALQSKLE ERFGPDSKSS ALACTLPTLP AKVYVGVKQE
预测反应 Cow: 85%, Dog: 86%, Guinea Pig: 79%, Horse: 86%, Human: 100%, Mouse: 92%, Pig: 92%, Rabbit: 85%, Rat: 92%
产品特性 This is a rabbit polyclonal antibody against NCF4. It was validated on Western Blot using a cell lysate as a positive control.
纯化方法 Affinity Purified
别名 NCF4 (NCF4 Antibody 摘要)
背景 NCF4 is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Alias Symbols: MGC3810, NCF, P40PHOX, SH3PXD4
Protein Interaction Partner: NCF2, UBC, CORO1A, MSN, CYBB, NCF1, CYBA, TXN, PRKCD, XRCC6, PRKDC,
Protein Size: 348
分子量 39 kDa
基因ID 4689
NCBI登录号 NM_013416, NP_038202
UniProt A8K4F9
研究领域 Metabolism
应用备注 Optimal working dilutions should be determined experimentally by the investigator.
说明

Antigen size: 348 AA

限制 仅限研究用
状态 Liquid
浓度 Lot specific
缓冲液 Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -20 °C
储存方法 For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
厂商提供的图像
Western Blotting (WB) image for anti-Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (N-Term) 抗体 (ABIN2782428) anti-Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (N-Term) antibody
背景 Chen, He, Minshall, Dinauer, Ye: "Characterization of a mutation in the Phox homology domain of the NADPH oxidase component p40phox identifies a mechanism for negative regulation of superoxide production." in: The Journal of biological chemistry, Vol. 282, Issue 41, pp. 30273-84, 2007 (PubMed).