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C10orf2 抗体 (Middle Region)

C10ORF2 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 马, 犬, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN2775251
发货至: 中国
  • 抗原 See all C10orf2 (C10ORF2) 抗体
    C10orf2 (C10ORF2) (Chromosome 10 Open Reading Frame 2 (C10ORF2))
    抗原表位
    • 9
    • 8
    • 6
    • 6
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Middle Region
    适用
    • 41
    • 13
    • 11
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    人, 小鼠, 大鼠, Cow, 豚鼠, 马, 犬, 兔, 斑马鱼
    宿主
    • 40
    • 2
    克隆类型
    • 40
    • 2
    多克隆
    标记
    • 15
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This C10orf2 antibody is un-conjugated
    应用范围
    • 25
    • 19
    • 17
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB)
    序列
    GVFRKFATDN NCHVTLVIHP RKEDDDKELQ TASIFGSAKA SQEADNVLIL
    预测反应
    Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%
    产品特性
    This is a rabbit polyclonal antibody against PEO1. It was validated on Western Blot using a cell lysate as a positive control.
    纯化方法
    Affinity Purified
    免疫原
    The immunogen is a synthetic peptide directed towards the middle region of human PEO1
  • 应用备注
    Optimal working dilutions should be determined experimentally by the investigator.
    说明

    Antigen size: 684 AA

    限制
    仅限研究用
  • 生效 #100038 (Western Blotting)
    '独立验证'标志
    by
    Mitochondrial Biology, University of Eastern Finland
    No.
    #100038
    日期
    2016.08.08
    抗原
    PEO1 antibody - middle region
    Lot Number
    QC6322
    Method validated
    Western Blotting
    Positive Control
    HeLa cells, HeLa cells overexpressing PEO1
    Negative Control
    rho0 HeLa cells
    Notes
    Passed, regarding sensitivity and specificity. The antibody detects an antigen at the expected apparent molecular weight of its antigen.
    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    ABIN2775251
    Secondary Antibody
    Goat-anti rabbit IgG (H+L), HRP-linked (Invitrogen, A-16104)
    Full Protocol
    • Cell lysates were propared from HeLa cells, HeLa cells overexpressing PEO1, and rho0 HeLa cells.
    • 75µg total protein or each sample were separated on a denaturing 8% SDS-PAGE gel (Laemmli 1970).
    • Immunoblot onto supported nitrocellulose membrane (Protran, GE Healthcare No. 1060005, 0.2µm) (Towbin et al., 1979).
    • Blocking of the membrane in 3% skim milk in TBST (50mM Tris-HCl, pH 7.4, 150mM NaCl, 0.1% Tween 20) for 1h at room temperature.
    • Incubation with primary antibody ABIN2775251 diluted 1:1000 in 3% BSA in TBST at 4°C overnight.
    • Washing in TBST for 3x 5min.
    • Incubation with goat-anti rabbit IgG (H+L), HRP-linked (Invitrogen, A-16104) diluted 1:15000 in 3% BSA in TBST for 1h at room temperature.
    • Washing in TBST for 5x 10min.
    • Chemiluminescence detection with luminol solution (250µg/ml Na-Luminol, 0,01% H2O2, 11µg/ml p-hydroxy-coumaric acid in 100mM Tris pH 8.5) and image capture on Kodak ECL film.
    Experimental Notes
    PEO1 runs usually at 95-100 kDa, although the theoretical size is 72 kDa, often it is also visible as dimer.
  • 生效 #100039 (Immunocytochemistry)
    '独立验证'标志
    by
    Mitochondrial Biology, University of Eastern Finland
    No.
    #100039
    日期
    2016.08.08
    抗原
    PEO1 antibody - middle region
    Lot Number
    QC6322
    Method validated
    Immunocytochemistry
    Positive Control
    Mouse-anti-ds DNA antibody (clone 35I9)
    Negative Control
    Notes
    Passed, regarding sensitivity and specificity. The antibody shows a staining pattern consistent with the expected mitochondrial localization of PEO1.
    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    ABIN2775251
    Secondary Antibody
    Goat-anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate (Invitrogen, A-11034, Lot 702323)
    Full Protocol
    • HeLa cells were grown in DMEM high glucose + 10% FBS on acid-cleaned glass coverslips to 50% confluency.
    • Cells were fixed in 4% para-formaldehyde in growth medium for 25min at RT, then washed once with growth medium and twice with PBS.
    • Permeabilization was achieved by incubation with 0.5% Triton X-100, 10% fetal bovine serum in PBS for 15min.
    • Primary antibodies:
      • ABIN2775251 diluted 1:200 in PBS with 0.1% Triton X-100 , 10% fetal bovine serum.
      • Co-staining of mitochondrial DNA with monoclonal antibody mouse-anti-dsDNA clone 35I9 DNA (Abcam ab27156, Lot GR27035-10) diluted 1:400 in PBS with 0.1% Triton X-100, 10% fetal bovine serum.
    • Incubation for 1h at RT.
    • Wash 3x 5min with PBS.
    • Secondary antibodies:
      • Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate (Invitrogen, A-11034, Lot 702323) diluted 1:1000 in PBS with 0,1% Triton X-100 , 10% fetal bovine serum, 1µM DAPI.
      • Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 594 conjugate (Invitrogen, A-11032, Lot 621333).
    • Incubation for 1h at RT.
    • Wash 3x 5min with PBS.
    • The coverslips were mounted with Immu-mount mounting medium (Thermo Fisher, 9990402) and imaged with a 630 x magnification on a Zeiss Axioplan 2 fluorescent microscope using an Axiocam camera and Axiovision 4.8 software.
    • For visualization of PEO1 the excitation wavelength was 488nm and emission wavelength >535nm, exposure time 53ms. DNA was visualized by excitation at 580nm and emission at >590nm, exposure time 410ms.
    Experimental Notes
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -20 °C
    储存方法
    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Wang, Xu, Alontaga, Chen, Liu: "Impaired p32 regulation caused by the lymphoma-prone RECQ4 mutation drives mitochondrial dysfunction." in: Cell reports, Vol. 7, Issue 3, pp. 848-58, (2014) (PubMed).

    Ylikallio, Tyynismaa, Tsutsui, Ide, Suomalainen: "High mitochondrial DNA copy number has detrimental effects in mice." in: Human molecular genetics, Vol. 19, Issue 13, pp. 2695-705, (2010) (PubMed).

  • 抗原
    C10orf2 (C10ORF2) (Chromosome 10 Open Reading Frame 2 (C10ORF2))
    别名
    PEO1 (C10ORF2 产品)
    别名
    ATXN8 antibody, IOSCA antibody, MTDPS7 antibody, PEO antibody, PEO1 antibody, PEOA3 antibody, SANDO antibody, SCA8 antibody, TWINL antibody, C6H10orf2 antibody, D19Ertd626e antibody, Twinl antibody, twinkle mtDNA helicase L homeolog antibody, twinkle mtDNA helicase antibody, twnk.L antibody, TWNK antibody, Twnk antibody
    背景
    Twinkle is a mitochondrial protein with structural similarity to the phage T7 primase/helicase (GP4) and other hexameric ring helicases. The twinkle protein colocalizes with mtDNA in mitochondrial nucleoids, and its name derives from the unusual localization pattern reminiscent of twinkling stars.Twinkle is a mitochondrial protein with structural similarity to the phage T7 primase/helicase (GP4) and other hexameric ring helicases. The twinkle protein colocalizes with mtDNA in mitochondrial nucleoids, and its name derives from the unusual localization pattern reminiscent of twinkling stars (Spelbrink et al., 2001 [PubMed 11431692]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-213 BG473173.1 6-218 214-1706 BX640829.1 215-1707 1707-3630 BC033762.1 719-2642
    Alias Symbols: C10orf2, FLJ21832, PEO, PEOA3, SANDO, TWINL, PEO1, SCA8, ATXN8, IOSCA, MTDPS7
    Protein Interaction Partner: ZBTB1, BMI1, SMAD9, ICT1, SQSTM1, AKTIP,
    Protein Size: 684
    分子量
    77 kDa
    基因ID
    56652
    NCBI登录号
    NM_021830, NP_068602
    UniProt
    Q96RR1
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