SRF (N-Term) 抗体

ABIN2774664 产品详细信息, 供应商: Log in to see
抗原
  • AW049942
  • AW240594
  • MCM1
  • SRFX
  • XSRF
  • srf-A
  • RGD1559787
  • srf
  • srf1
  • serum response factor
  • serum response factor (c-fos serum response element-binding transcription factor)
  • serum response factor a
  • Srf
  • SRF
  • srf
  • srfa
抗原表位
N-Term
23
21
7
6
6
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
适用
牛, 犬, 人, 小鼠, 猪, 兔, 大鼠, 斑马鱼
155
112
78
5
4
4
3
2
2
1
1
1
1
1
宿主

125
17
14
2
克隆类型
多克隆
标记
非结合性
4
4
4
3
3
3
3
3
3
3
3
3
3
3
1
1
1
应用范围
Chromatin Immunoprecipitation (ChIP), Western Blotting (WB)
112
52
45
39
30
8
7
7
3
2
2
2
1
1
1
选项
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免疫原 The immunogen is a synthetic peptide directed towards the N terminal region of human SRF
序列 ATGGYGPVSG AVSGAKPGKK TRGRVKIKME FIDNKLRRYT TFSKRKTGIM
交叉反应 牛, 犬, 小鸡, 人, 小鼠, 大鼠
预测反应 Cow: 100%, Dog: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%
产品特性 This is a rabbit polyclonal antibody against SRF. It was validated on Western Blot using a cell lysate as a positive control.
纯化方法 Protein A purified
别名 Serum response factor (SRF)
背景 SRF is a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways, for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs).
Alias Symbols: MCM1
Protein Interaction Partner: Camk2d, ZIC3, ELK1, SRFBP1, SRF, NFKB1, PRKDC, CIRBP, ALDH3A1, SUMO1, UBE2I, SHBG, TRIM63, KDM6A, MYOCD, MKL1, CNTN2, CSNK2A1, SMARCA4, PELP1, KAT5, HDAC4, EPC1, SMAD7, HDAC3, MKL2, ATF6, BARX2, MAPKAPK2, FHL3, GATA4, CAMK2A, HOPX, PYCARD, NKX2-3, MINPP1,
Protein Size: 508
分子量 52 kDa
基因ID 6722
NCBI登录号 NM_003131, NP_003122
UniProt P11831
应用备注 WB Suggested Anti-SRF Antibody Titration: 1.25 µg/mL
ELISA Titer: 1:1562500
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.
说明

Antigen size: 508 AA

限制 仅限研究用
状态 Liquid
浓度 Lot specific
缓冲液 Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -20 °C
储存方法 For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
厂商提供的图像
Western Blotting (WB) image for anti-SRF (N-Term) antibody (ABIN2774664) WB Suggested Anti-SRF Antibody Titration: 1.25ug/ml
ELISA Titer: 1:1...
Western Blotting (WB) image for anti-SRF (N-Term) antibody (ABIN2774664) WB Suggested Anti-SRF
Antibody Titration: 1.25 µg/mL ELISA Titer: 1:1562500
Chromatin Immunoprecipitation (ChIP) image for anti-SRF (N-Term) antibody (ABIN2774664) Chromatin Immunoprecipitation (ChIP) Using SRF Antibody - N-terminal region (P100866_...
背景 Doi, Iso, Yamazaki, Akiyama, Kanai, Sato, Kawai-Kowase, Tanaka, Maeno, Okamoto, Arai, Kedes, Kurabayashi: "HERP1 inhibits myocardin-induced vascular smooth muscle cell differentiation by interfering with SRF binding to CArG box." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 25, Issue 11, pp. 2328-34, 2005 (PubMed).

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