(6 references)-
- 抗原 See all RUNX3 抗体
- RUNX3 (Runt-Related Transcription Factor 3 (RUNX3))
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适用
- 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This RUNX3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Intracellular Staining (ICS)
- 品牌
- BD Pharmingen™
- 产品特性
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The R3-5G4 monoclonal antibody specifically binds to Runt-related transcription factor 3 (RUNX3) which is also known as Acute myeloid leukemia 2 protein (AML2), Core-binding factor subunit alpha-3 (CBFA3), and Polyomavirus enhancer-binding protein 2 alpha C subunit (PEBP2aC). RUNX3 is a member of the RUNX transcription factor family which includes RUNX1-3. RUNX3 is a key regulator of gene expression related to the development and differentiation of cells within the nervous and immune systems. In the immune system, RUNX3 is particularly involved in the commitment of CD8+ T cells in the thymus. RUNX3 is highly expressed and essential for the cytotoxic functions of NK cells, peripheral CD8+T cells and CD4+CD8αα intraepithelial lymphocytes in the gut. RUNX3 also plays a role in the differentiation and effector functions of Th1 cells. RUNX3 is activated downstream of the TGF-β signaling pathway and can play a role in tumor suppression. Aberrant expression of RUNX3 has been associated with tumorigenesis including the development of gastric and other cancers. Western blot and Immunohistochemical analyses of RUNX3 expression. Left Panel: Western blot analysis of RUNX3 expression in Jurkat cell lysate. Lysate was prepared from cells of the human Jurkat (Acut T cell leukemia, ATCC TIB-152) cell line. Cell lysate proteins were resolved by electrophoresis (SDS-PAGE) in a 4-20 % Tris-Glycine polyacrylamide gel, transferred to PVDF membranes and then probed with 1 μg/mL of Purified Anti-RUNX3 antibody (Cat. No. 564813) followed by HRP-conjugated Goat Anti-Mouse IgG (Cat. No. 554002), and ECL Western blot detection reagents. RUNX3 was identified as protein bands of approximately 40 to 50 kDa. Right Panel: Immunohistochemical staining of RUNX3 expressed in human tonsillar cells. Following antigen retrieval with BD Retrievagen A Buffer (Cat. No. 550524), the formalin-fixed paraffin-embedded tonsil sections were stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No. 550878, Left Image) or Purified Mouse Anti-RUNX3 antibody (Cat. No.564813, Right Image). A three-step staining procedure that employs a Biotin Goat Anti-Mouse Immunoglobulin (Cat. No. 550337), Streptavidin-Horseradish Peroxidase (HRP) (Cat. No.550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. As shown in the Right Image, the RUNX3-specific antibody primarily stained the nuclei of some tonsillar lymphocytes. Original magnification: 40x.
BD Pharmingen™ Purified Mouse Anti-RUNX3 - Purified - Clone R3-5G4 - Isotype Mouse IgG1, κ - Reactivity Hu, Ms - 0.1 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human RUNX3 Recombinant Protein
- 克隆位点
- R3
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product RUNX3 Primary Antibody
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anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 31-130) antibody
RUNX3 适用: 人 WB, ELISA, IF (p), FACS, IF (cc), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 1-429) antibodyRUNX3 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 294-429) antibodyRUNX3 适用: 人 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 7E2B8 unconjugated
anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 128-270) antibodyRUNX3 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 186-252) antibodyRUNX3 适用: 人, 小鼠 WB, ELISA, IHC, FACS, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 1-429) antibodyRUNX3 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 168-197) antibodyRUNX3 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 1-415) antibodyRUNX3 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 194-279) antibodyRUNX3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Runt-Related Transcription Factor 3 (RUNX3) (Internal Region) antibodyRUNX3 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "RUNX family: Regulation and diversification of roles through interacting proteins." in: International journal of cancer. Journal international du cancer, Vol. 132, Issue 6, pp. 1260-71, (2013) (PubMed).
: "Runx3-mediated transcriptional program in cytotoxic lymphocytes." in: PLoS ONE, Vol. 8, Issue 11, pp. e80467, (2013) (PubMed).
: "Mutual expression of the transcription factors Runx3 and ThPOK regulates intestinal CD4⁺ T cell immunity." in: Nature immunology, Vol. 14, Issue 3, pp. 271-80, (2013) (PubMed).
: "Regulation of gene expression in peripheral T cells by Runx transcription factors." in: Advances in immunology, Vol. 104, pp. 1-23, (2010) (PubMed).
: "Repression of the transcription factor Th-POK by Runx complexes in cytotoxic T cell development." in: Science (New York, N.Y.), Vol. 319, Issue 5864, pp. 822-5, (2008) (PubMed).
: "RUNX3, a novel tumor suppressor, is frequently inactivated in gastric cancer by protein mislocalization." in: Cancer research, Vol. 65, Issue 17, pp. 7743-50, (2005) (PubMed).
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: "RUNX family: Regulation and diversification of roles through interacting proteins." in: International journal of cancer. Journal international du cancer, Vol. 132, Issue 6, pp. 1260-71, (2013) (PubMed).
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- 抗原
- RUNX3 (Runt-Related Transcription Factor 3 (RUNX3))
- 别名
- RUNX3 (RUNX3 产品)
- 别名
- runxb antibody, RUNX3 antibody, CBFA3 antibody, frrunx3 antibody, runx3 antibody, aml2 antibody, cbfa3 antibody, pebp2ac antibody, AML2 antibody, Cbfa3 antibody, Pebp2a3 antibody, PEBP2aC antibody, runt-related transcription factor 3 antibody, runt related transcription factor 3 antibody, runt related transcription factor 3 L homeolog antibody, runx3 antibody, RUNX3 antibody, runx3.L antibody, Runx3 antibody
- 背景
- Synonyms: AML2, AML-2, CBFA3, PEBP2aC, PEBP2A3
- 分子量
- 40-50 kDa
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