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CD8 alpha 抗体 (Biotin)

Name: CD8 alpha 抗体 (Biotin)
SKU: ABIN2689436
Availability: OutOfStock

CD8A 适用: 小鼠 FACS, Sep 宿主: 大鼠 Monoclonal 53 Biotin

(22 references)

产品编号 ABIN2689436

发货至: 中国

Datasheet as PDF

抗原 See all CD8 alpha (CD8A) 抗体

CD8 alpha (CD8A) (CD8a Molecule (CD8A))
适用
193

119

40

37

11

7

3

2

2

2

2

2

2

2

2

1

1
小鼠
宿主
144

130

100

1
大鼠
Compatible Secondaries
克隆类型
271

100

1
单克隆
标记
132

43

30

26

25

8

6

6

6

6

5

5

5

4

4

4

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD8 alpha antibody is conjugated to Biotin
应用范围
288

106

93

66

54

46

42

24

15

15

14

9

8

7

6

6

6

5

3

3

2

2

1

1

1

1

1

1
Flow Cytometry (FACS), Separation (Sep)

品牌

BD Pharmingen™

产品特性

The 53-6.7 antibody has been reported to react with the 38 kDa α and 34 kDa α' chains of the CD8 differentiation antigen (Ly-2 or Lyt-2) of all mouse strains tested. The CD8 α and α' chains (CD8a) form heterodimers with the CD8 β chain (CD8b, Ly-3, or Lyt-3) on the surface of most thymocytes. A subpopulation of mature T lymphocytes (i.e., MHC class I-restricted T cells, including most T suppressor/cytotoxic cells) expresses almost exclusively the CD8 αβ heterodimer (the α' chain is absent). Subsets of γδ TCR-bearing T cells, intestinal intrapithelial lymphocytes, and dendritic cells express CD8a without CD8b. It has been suggested that the expression of the CD8a/CD8b heterodimer is restricted to T lymphocytes which matured in the thymus or in an extrathymic environment that had been influenced by thymus-initiated neuroendocrine signals. CD8 is an antigen coreceptor on the T-cell surface which interacts with MHC class I molecules on antigen-presenting cells or epithelial cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck (p56 [lck]). The CD8 α and α' chains arise from alternatively spliced messengers of a single CD8a gene. The longer α form associates with p56 [lck] via a CXCP motif in its cytoplasmic domain, which it shares with CD4, but not with CD8b. The truncated α' chain is unable to associate with p56 [lck], and it may function to attenuate the CD8-mediated costimulatory signal during intrathymic T-cell maturation. In vivo and in vitro treatment with 53-6.7 mAb has reportedly been effective at depleting CD8+ peripheral T lymphocytes. The 53-6.7 antibody has also been reported to cross-reaact with CD8 α- and α'-like polypeptides on subsets of thymic and peripheral lymphocytes in the Egyptian toad, Bufo regularis. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Biotin Rat Anti-Mouse CD8a - Biotin - Clone 53-6.7 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Mouse thymus / spleen

克隆位点

53

亚型

IgG2a kappa

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CD8A 适用: 小鼠 FACS, IP, IHC (fro), IHC (p), ICC, Func 宿主: 大鼠 Monoclonal 53-6-7 unconjugated

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CD8A 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-CD8a Molecule (CD8A) (C-Term) antibody
CD8A 适用: 人 FACS, WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
浓度

0.5 mg/mL

缓冲液

Aqueous buffered solution containing ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

储存条件

4 °C

储存方法

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Leishman, Naidenko, Attinger, Koning, Lena, Xiong, Chang, Reinherz, Kronenberg, Cheroutre: "T cell responses modulated through interaction between CD8alphaalpha and the nonclassical MHC class I molecule, TL." in: Science (New York, N.Y.), Vol. 294, Issue 5548, pp. 1936-9, (2001) (PubMed).

Traver, Akashi, Manz, Merad, Miyamoto, Engleman, Weissman: "Development of CD8alpha-positive dendritic cells from a common myeloid progenitor." in: Science (New York, N.Y.), Vol. 290, Issue 5499, pp. 2152-4, (2000) (PubMed).

Mitnacht, Bischof, Torres-Nagel, Hünig: "Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 2, pp. 700-7, (1998) (PubMed).

Negm, Mansour, Saad, Abdel Halim: "Structural characterization of an Lyt-2/3 homolog expressed on Bufo regularis lymphocytes." in: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, Vol. 113, Issue 1, pp. 79-87, (1997) (PubMed).

Fujiura, Kawaguchi, Kondo, Obana, Yamamoto, Nanno, Ishikawa: "Development of CD8 alpha alpha+ intestinal intraepithelial T cells in beta 2-microglobulin- and/or TAP1-deficient mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 8, pp. 2710-5, (1996) (PubMed).

Sydora, Brossay, Hagenbaugh, Kronenberg, Cheroutre: "TAP-independent selection of CD8+ intestinal intraepithelial lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 11, pp. 4209-16, (1996) (PubMed).

Zamoyska: "The CD8 coreceptor revisited: one chain good, two chains better." in: Immunity, Vol. 1, Issue 4, pp. 243-6, (1995) (PubMed).

Nakayama, Nakayama, Negishi, Kuida, Louie, Kanagawa, Nakauchi, Loh: "Requirement for CD8 beta chain in positive selection of CD8-lineage T cells." in: Science (New York, N.Y.), Vol. 263, Issue 5150, pp. 1131-3, (1994) (PubMed).

Wang, Klein: "Thymus-neuroendocrine interactions in extrathymic T cell development." in: Science (New York, N.Y.), Vol. 265, Issue 5180, pp. 1860-2, (1994) (PubMed).

ORourke, Mescher: "The roles of CD8 in cytotoxic T lymphocyte function." in: Immunology today, Vol. 14, Issue 4, pp. 183-8, (1993) (PubMed).

Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, (1992) (PubMed).

Lefrançois: "Extrathymic differentiation of intraepithelial lymphocytes: generation of a separate and unequal T-cell repertoire?" in: Immunology today, Vol. 12, Issue 12, pp. 436-8, (1992) (PubMed).

Murosaki, Yoshikai, Ishida, Nakamura, Matsuzaki, Takimoto, Yuuki, Nomoto: "Failure of T cell receptor V beta negative selection in murine intestinal intra-epithelial lymphocytes." in: International immunology, Vol. 3, Issue 10, pp. 1005-13, (1992) (PubMed).

MacDonald, Schreyer, Howe, Bron: "Selective expression of CD8 alpha (Ly-2) subunit on activated thymic gamma/delta cells." in: European journal of immunology, Vol. 20, Issue 4, pp. 927-30, (1990) (PubMed).

Bierer, Sleckman, Ratnofsky, Burakoff: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, (1989) (PubMed).

Zamoyska, Derham, Gorman, von Hoegen, Bolen, Veillette, Parnes: "Inability of CD8 alpha' polypeptides to associate with p56lck correlates with impaired function in vitro and lack of expression in vivo." in: Nature, Vol. 342, Issue 6247, pp. 278-81, (1989) (PubMed).

Zamoyska, Vollmer, Sizer, Liaw, Parnes: "Two Lyt-2 polypeptides arise from a single gene by alternative splicing patterns of mRNA." in: Cell, Vol. 43, Issue 1, pp. 153-63, (1986) (PubMed).

Walker, Murray, Hogarth, Kelso, McKenzie: "Comparison of thymic and peripheral T cell Ly-2/3 antigens." in: European journal of immunology, Vol. 14, Issue 10, pp. 906-10, (1984) (PubMed).

van Ewijk, van Soest, van den Engh: "Fluorescence analysis and anatomic distribution of mouse T lymphocyte subsets defined by monoclonal antibodies to the antigens Thy-1, Lyt-1, Lyt-2, and T-200." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 127, Issue 6, pp. 2594-604, (1982) (PubMed).

Ledbetter, Seaman, Tsu, Herzenberg: "Lyt-2 and lyt-3 antigens are on two different polypeptide subunits linked by disulfide bonds. Relationship of subunits to T cell cytolytic activity." in: The Journal of experimental medicine, Vol. 153, Issue 6, pp. 1503-16, (1981) (PubMed).

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抗原

CD8 alpha (CD8A) (CD8a Molecule (CD8A))

别名

CD8a (CD8A 产品)

别名

cd8 antibody, LOC100136450 antibody, BB154331 antibody, Ly-2 antibody, Ly-35 antibody, Ly-B antibody, Lyt-2 antibody, zgc:136643 antibody, CD8 antibody, Leu2 antibody, MAL antibody, p32 antibody, RHACD8-4 antibody, CD8a molecule antibody, uncharacterized LOC100125537 antibody, CD8 alpha antibody, CD8 antigen, alpha chain antibody, T-cell surface glycoprotein CD8 alpha chain antibody, Cd8a antibody, LOC100125537 antibody, LOC100136450 antibody, CD8A antibody, cd8a antibody, LOC100126556 antibody

背景

Synonyms: Ly-2, Lyt-2

途径

TCR Signaling