The 10E9.6 antibody reacts with the 97-110 kDa cell surface glycoprotein E-selectin (CD62E), also known as endothelial-leukocyte adhesion molecule-1 (ELAM-1), which is expressed on endotoxin- or cytokine-stimulated mouse endothelial cells. A suspension of TNFα stimulated mouse brain capillary endothelioma cells, from the cell line bEnd.3, was used as the immunogen. The epitope recognized by mAb 10E9.6 has been mapped to the first and/or second complement regulatory protein repeat domains of E-selectin. The 10E9.6 antibody has been reported to block binding of a monocyte cell line to E-selectin in vitro and to block neutrophil migration in BALB/c, but not C57BL/6 mice. It has no effect on leukocyte rolling in TNFα-treated mouse venules or on in vitro adhesion of myeloid cells to E-selectin. Studies have demonstrated that Cutaneous Lymphocyte Antigen (CLA), recognized by mAb HECA-452 (Cat. no. 555946), may be a ligand for CD62E. Immunohistochemical staining for CD62E. Frozen tongue sections of a lipopolysaccharide treated C57BL/6 mouse was reacted with the 10E9.6 antibody. Endothelial cells of the blood vessels express CD62E and can be identifed by the brown staining.
BD Pharmingen™ Purified Rat Anti-Mouse CD62E - Purified - Clone 10E9.6 - Isotype Rat IgG2a, κ - Reactivity Ms - 1 mL
纯化方法
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C
储存方法
Store undiluted at 4°C.
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Eppihimer, Wolitzky, Anderson, Labow, Granger: "Heterogeneity of expression of E- and P-selectins in vivo." in: Circulation research, Vol. 79, Issue 3, pp. 560-9, (1996) (PubMed).
Ley, Bullard, Arbonés, Bosse, Vestweber, Tedder, Beaudet: "Sequential contribution of L- and P-selectin to leukocyte rolling in vivo." in: The Journal of experimental medicine, Vol. 181, Issue 2, pp. 669-75, (1995) (PubMed).