(3 references)-
- 抗原 See all ITGA2 抗体
- ITGA2 (Integrin alpha 2 (ITGA2))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This ITGA2 antibody is conjugated to Biotin
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 品牌
- BD Pharmingen™
- 产品特性
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The rat anti-mouse CD49b monoclonal antibody (clone DX5) specifically binds to the integrin α2 chain (CD49b). CD49b is a 150 kDa transmembrane glycoprotein that non-covalently associates with CD29 (integrin β1) to form the integrin α2β1 complex known as VLA-2. The rat anti-mouse CD49b antibody (clone DX5) has been reported to identify the majority of NK cells and a small T-cell subpopulation in most mouse strains (e.g., A/J, AKR, BALB/c, C3H/HeJ, C57BL/6, C57BL/10, C57BR, C58, CBA/Ca, DBA/1, DBA/2, SJL, SWR, 129/J, but not NOD). The DX5 antibody also recognizes platelets that express high levels of CD49b. Multiparameter flow cytometric analysis has demonstrated that most lymphocytes which express NK-1.1 (NKR-P1B and NKR-P1C), as detectable by mouse anti-mouse NK-1.1 antibody (clone PK136), also express the DX5 antigen. Small DX5+ NK-1.1- and DX5- NK-1.1+ cell subsets are found, especially among the CD3-positive cell population. Some CD49b+ NK cells have been reported to gradually lose reactivity with the rat anti-mouse CD49b antibody (clone DX5) when cultured in the presence of recombinant human IL-2. The resulting DX5-negative cells have weakened cytotoxic activity when compared to the remaining DX5+ cells. This indicates that the DX5 antibody distinguishes functional subsets of NK cells. No activation or blocking activity of the rat anti-mouse antibody (clone DX5) has been observed. Staining of splenic NK cells with this antibody reportedly can be blocked by hamster anti-mouse CD49b antibody (clone HMα2). Detection of NK cells with monoclonal antibodies. Freshly isolated splenocytes from a C57BL/6 mouse were simultaneously stained with FITC Mouse anti-Mouse NK-1.1 antibody (Cat. No. 553164, both panels) and Biotin Rat anti-Mouse CD49b antibody (clone DX5) (right panel) followed by PE-Streptavidin (Cat. No. 554061, both panels). Flow cytometry was performed on a BD FACScan™ instrument .
BD Pharmingen™ Biotin Rat Anti-Mouse CD49b - Biotin - Clone DX5 - Isotype Rat IgM, κ - Reactivity Ms - 0.5 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Mouse (C57BL/6) NK1.1+ cells propagated with rIL-2
- 克隆位点
- DX5
- 亚型
- IgM kappa
- Top Product
- Discover our top product ITGA2 Primary Antibody
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anti-Integrin alpha 2 (ITGA2) (AA 30-119) antibody
ITGA2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2B6 unconjugated
anti-Integrin alpha 2 (ITGA2) (AA 994-1132) antibodyITGA2 适用: 人 FACS, WB, IHC, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 8A11A2 unconjugated
anti-Integrin alpha 2 (ITGA2) (AA 170-361) antibodyITGA2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 2 (ITGA2) antibodyITGA2 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 2 (ITGA2) (Internal Region) antibodyITGA2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 2 (ITGA2) (C-Term) antibodyITGA2 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 2 (ITGA2) (AA 678-894) antibodyITGA2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 2 (ITGA2) (AA 74-277) antibodyITGA2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 2 (ITGA2) (AA 188-365) antibodyITGA2 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 2 (ITGA2) (C-Term) antibodyITGA2 适用: 人, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "Cutting edge: the mouse NK cell-associated antigen recognized by DX5 monoclonal antibody is CD49b (alpha 2 integrin, very late antigen-2)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 3, pp. 1141-4, (2001) (PubMed).
: "The Ly-49 family: regulation of cytotoxicity and cytokine production in murine CD3+ cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 3, pp. 1158-65, (1998) (PubMed).
: "Inhibition of gamma delta T cell development and early thymocyte maturation in IL-7 -/- mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 6, pp. 2366-73, (1996) (PubMed).
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: "Cutting edge: the mouse NK cell-associated antigen recognized by DX5 monoclonal antibody is CD49b (alpha 2 integrin, very late antigen-2)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 3, pp. 1141-4, (2001) (PubMed).
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- 抗原
- ITGA2 (Integrin alpha 2 (ITGA2))
- 别名
- CD49b (ITGA2 产品)
- 别名
- BR antibody, CD49B antibody, GPIa antibody, HPA-5 antibody, VLA-2 antibody, VLAA2 antibody, DX5 antibody, integrin subunit alpha 2 antibody, integrin alpha 2 antibody, ITGA2 antibody, Itga2 antibody
- 背景
- Synonyms: itga2, Integrin alpha-2, DX5, Pan NK cell marker, VLAA2, VLA-2 alpha chain
- 途径
- CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Integrin Complex
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