(8 references)-
- 抗原 See all FZD4 抗体
- FZD4 (Frizzled Family Receptor 4 (FZD4))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This FZD4 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 品牌
- BD Pharmingen™
- 产品特性
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The CH3A4A7 monoclonal antibody reacts with an extracellular epitope on human CD344 which is also known as Frizzled family receptor 4 (FZD4) or Frizzled-4. CD344 is a member of the Frizzled family of proteins within the G protein-coupled receptor (GPCR) superfamily. CD344 is a receptor for Wnt proteins and participates in the activation of the Wnt/beta-catenin pathway (canonical Wnt signaling). Patients with familial exudative vitreoretinopathy (FEVR) carry mutations in Frizzled-4. This disease is characterized by retinal degeneration and hearing loss. On tissues, CD344 is almost ubiquitously expressed. CD344 is highly expressed in adult heart and skeletal muscle. Flow Cytometric and Immunohistochemical Analysis of CD344 Expression. Panel A: Flow cytometric analysis of CD344 expression on human cell lines. Cells from the Weri-RB-1 Retinoblastoma Cell Line (ATCC HTB-169, solid line histogram) and Daudi Burkitt's Lymphoma Cell Line (ATCC CCL-213, dashed line histogram) were stained with Purified Mouse Anti-Human CD344 antibody (Cat. No. 563657). The cells were then washed and stained with PE Polyclonal Goat Anti-Mouse Ig (Cat. No.550589). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System. Panel B: Immunohistochemical analysis of CD344 expression by cardiac muscle. Acetone-fixed, frozen human heart sections were stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No.550878, Left Image) or Purified Mouse Anti-Human CD344 antibody (Right Image). A three-step staining procedure that employed Biotin Goat Anti-Mouse Immunoglobulin (Cat. No. 550337), Streptavidin-Horseradish Peroxidase (HRP) (Cat. No. 550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. Original magnification:40x.
BD Pharmingen™ Purified Mouse Anti-Human CD344 - Purified - Clone CH3A4A7 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human neuroblastoma Cell Line
- 克隆位点
- CH3A4A7
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product FZD4 Primary Antibody
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anti-Frizzled Family Receptor 4 (FZD4) (AA 37-222) antibody
FZD4 适用: 人 WB, ELISA, IHC, FACS, ICC, Neut 宿主: 小鼠 Monoclonal 2F3A7 unconjugated
anti-Frizzled Family Receptor 4 (FZD4) (Middle Region) antibodyFZD4 适用: 人, 小鼠, 大鼠, Cow, 马, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 4 (FZD4) (AA 11-60) antibodyFZD4 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 4 (FZD4) (AA 37-222) antibodyFZD4 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 4 (FZD4) (AA 100-200) antibody (Atto 594)FZD4 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal Atto 594
anti-Frizzled Family Receptor 4 (FZD4) (AA 100-200) antibody (Atto 488)FZD4 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal Atto 488
anti-Frizzled Family Receptor 4 (FZD4) (AA 100-200) antibody (Atto 390)FZD4 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal Atto 390
anti-Frizzled Family Receptor 4 (FZD4) (AA 107-206) antibodyFZD4 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3G7 unconjugated
anti-Frizzled Family Receptor 4 (FZD4) (AA 100-200) antibodyFZD4 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 4 (FZD4) (AA 100-200) antibody (HRP)FZD4 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal HRP
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Shared cell surface marker expression in mesenchymal stem cells and adult sarcomas." in: Stem cells translational medicine, Vol. 2, Issue 1, pp. 53-60, (2013) (PubMed).
: "Severe retinopathy of prematurity associated with FZD4 mutations." in: Ophthalmic genetics, Vol. 31, Issue 1, pp. 37-43, (2010) (PubMed).
: "Overview of the mutation spectrum in familial exudative vitreoretinopathy and Norrie disease with identification of 21 novel variants in FZD4, LRP5, and NDP." in: Human mutation, Vol. 31, Issue 6, pp. 656-66, (2010) (PubMed).
: "CD molecules 2006--human cell differentiation molecules." in: Journal of immunological methods, Vol. 319, Issue 1-2, pp. 1-5, (2007) (PubMed).
: "Expression pattern of Wnt signaling components in the adult intestine." in: Gastroenterology, Vol. 129, Issue 2, pp. 626-38, (2005) (PubMed).
: "Genetic variants of frizzled-4 gene in familial exudative vitreoretinopathy and advanced retinopathy of prematurity." in: Clinical genetics, Vol. 67, Issue 4, pp. 363-6, (2005) (PubMed).
: "Vascular development in the retina and inner ear: control by Norrin and Frizzled-4, a high-affinity ligand-receptor pair." in: Cell, Vol. 116, Issue 6, pp. 883-95, (2004) (PubMed).
: "Molecular cloning and characterization of human Frizzled-4 on chromosome 11q14-q21." in: Biochemical and biophysical research communications, Vol. 264, Issue 3, pp. 955-61, (1999) (PubMed).
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: "Shared cell surface marker expression in mesenchymal stem cells and adult sarcomas." in: Stem cells translational medicine, Vol. 2, Issue 1, pp. 53-60, (2013) (PubMed).
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- 抗原
- FZD4 (Frizzled Family Receptor 4 (FZD4))
- 别名
- CD344 (FZD4 产品)
- 别名
- CG4626 antibody, DFz4 antibody, Dfz4 antibody, Dm Fz4 antibody, Dmel\\CG4626 antibody, Fz4 antibody, anon-WO0170980.10 antibody, anon-WO0170980.11 antibody, fz1 antibody, zg01 antibody, CD344 antibody, EVR1 antibody, FEVR antibody, FZD4S antibody, Fz-4 antibody, FzE4 antibody, GPCR antibody, hFz4 antibody, frizzled4 antibody, fz4 antibody, FZ-4 antibody, frizzled 4 antibody, frizzled class receptor 4 antibody, frizzled-4 antibody, frizzled class receptor 4 S homeolog antibody, fz4 antibody, fzd4 antibody, Tsp_10376 antibody, FZD4 antibody, Fzd4 antibody, fzd4.S antibody
- 背景
- Synonyms: FZD4, Frizzled family receptor 4, Frizzled-4, Fz-4, FzE4, hFz4, EVR1, FEVR
- 途径
- WNT signaling, Hormone Transport, Sensory Perception of Sound
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