(4 references)-
- 抗原 See all NCR1 抗体
- NCR1 (Natural Cytotoxicity Triggering Receptor 1 (NCR1))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This NCR1 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS)
- 品牌
- BD Pharmingen™
- 产品特性
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The monoclonal antibody 29A1.4 specifically binds to mouse CD335, also known as NKp46. NKp46 is a 46 kDa type I transmembrane glycoprotein that is a member of the natural cytotoxicity receptor (NCR) family and immunoglobulin superfamily. NKp46 is encoded by the Ncr1 gene located on chromosome 7. NKp46 functions as a cytotoxicity triggering receptor and is selectively expressed by immature and mature NK cells in all mouse strains tested. NKp46 is detected on a minute fraction of NK-like T cells (less than 2 % of NKp46+ express CD3e) but not on CD1d-restricted NKT cells from C57BL/6 mice. When immobilized on tissue culture plates, the 29A1.4 antibody reportedly stimulates NK cells to produce interferon-gamma and to release their cytoplasmic granule contents. Although the ligands for the NKp46 receptor have not been fully characterized, recent evidence indicates that this receptor plays an important role in the NK cell-mediated recognition and killing of some virus-infected cells and tumor cells. The immunogen used to generate the 29A1.4 clone was mouse NKp46-Fc recombinant protein. Flow cytometric analysis of CD335 (NKp46) expression on mouse splenocytes. C57BL/6 and BALB/c mouse spleen cells were stained separately with Purified anti-mouse CD335 (NKp46) antibody followed by PE-conjugated Goat anti-rat Ig (Cat. No. 550767). After washing, C57BL/6 cells were stained with APC-conjugated anti-mouse NK-1.1 (NKR-P1B and NKR-P1C) antibody (Cat. No.550627, left panel) and BALB/c cells were stained with FITC-conjugated anti-mouse CD49b (DX5) antibody (Cat. No.553857, right panel). Two-color dot plots showing the correlated expression patterns of CD335/NKp46 and either NK-1.1/CD161 (C57BL/6 cells, left panel) or DX5/CD49b (BALB/c cells, right panel) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSRII System.
BD Pharmingen™ Purified Rat Anti-Mouse CD335 (NKp46) - Purified - Clone 29A1.4 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 克隆位点
- 29A1-4
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product NCR1 Primary Antibody
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anti-Natural Cytotoxicity Triggering Receptor 1 (NCR1) (AA 198-206) antibody
NCR1 适用: 人, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Natural Cytotoxicity Triggering Receptor 1 (NCR1) (AA 22-258) antibodyNCR1 适用: 人 WB, ELISA, FACS, IHC, ICC, Neut 宿主: 小鼠 Monoclonal 2G3C1 unconjugated
anti-Natural Cytotoxicity Triggering Receptor 1 (NCR1) (AA 17-258) antibodyNCR1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Natural Cytotoxicity Triggering Receptor 1 (NCR1) (Internal Region) antibodyNCR1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Natural Cytotoxicity Triggering Receptor 1 (NCR1) (AA 17-211) antibodyNCR1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Natural Cytotoxicity Triggering Receptor 1 (NCR1) (AA 121-230) antibodyNCR1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Natural Cytotoxicity Triggering Receptor 1 (NCR1) antibodyNCR1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Natural Cytotoxicity Triggering Receptor 1 (NCR1) (AA 22-258) antibodyNCR1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Natural Cytotoxicity Triggering Receptor 1 (NCR1) antibodyNCR1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Natural Cytotoxicity Triggering Receptor 1 (NCR1) antibodyNCR1 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal n1D9 unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "NK cell responsiveness is tuned commensurate with the number of inhibitory receptors for self-MHC class I: the rheostat model." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 8, pp. 4572-80, (2009) (PubMed).
: "Identification, activation, and selective in vivo ablation of mouse NK cells via NKp46." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 9, pp. 3384-9, (2007) (PubMed).
: "Lethal influenza infection in the absence of the natural killer cell receptor gene Ncr1." in: Nature immunology, Vol. 7, Issue 5, pp. 517-23, (2006) (PubMed).
: "The murine homologue of the human NKp46, a triggering receptor involved in the induction of natural cytotoxicity." in: European journal of immunology, Vol. 29, Issue 3, pp. 1014-20, (1999) (PubMed).
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: "NK cell responsiveness is tuned commensurate with the number of inhibitory receptors for self-MHC class I: the rheostat model." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 8, pp. 4572-80, (2009) (PubMed).
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- 抗原
- NCR1 (Natural Cytotoxicity Triggering Receptor 1 (NCR1))
- 别名
- CD335 NKp46 (NCR1 产品)
- 别名
- CD335 antibody, LY94 antibody, NK-p46 antibody, NKP46 antibody, Ly94 antibody, NKp46 antibody, KILR-1 antibody, Nk-p46 antibody, Nkp46 antibody, natural cytotoxicity triggering receptor 1 antibody, NCR1 antibody, Ncr1 antibody
- 背景
- Synonyms: NKp46, Ar1, Ly94, Lymphocyte antigen 94, Mar1, MAR-1, Mouse activating rece
- 途径
- Regulation of Leukocyte Mediated Immunity
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