(5 references)-
- 抗原 See all TRAIL (TNFSF10) 抗体
- TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This TRAIL antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Biochemical Assay (BCA), Blocking Reagent (BR)
- 品牌
- BD Pharmingen™
- 产品特性
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The RIK-2 monoclonal antibody specifically binds to CD253 which is also known as TRAIL (TNF-Related Apoptosis-Inducing Ligand) and Apo2 ligand (APO-2L). CD253 is a member of the Tumor Necrosis Factor Superfamily and is encoded by the TNFSF10 gene. CD253 is a type II membrane protein that may be expressed as a full-length, cell surface associated protein as well as in a soluble form. Both surface and soluble forms of TRAIL rapidly induce apoptosis by a wide range of cell lines but not by normal cells. TRAIL is expressed by activated T cells, NK cells, dendritic cells, monocytes and by a variety of non-lymphoid cells. TRAIL can bind to and exert apoptosis through DR4 (TRAIL-R1) and DR5 (TRAIL-R2) receptors. It can also bind to decoy receptors, including DcR1/TRID/TRAIL-R3 and DcR2/TRUNDD/TRAIL-R4, and possibly OPG/TNFRSF11B, which may serve to regulate TRAIL activity. TRAIL has been shown to be involved in T cell-mediated cytotoxicity, however its mechanism of action remains to be fully elucidated. The RIK-2 clone was selected based on its ability to block TRAIL-mediated cytotoxic activity. Flow Cytometric Analysis of TRAIL-induced Killing and RIK-2 Blocking using FITC Annexin V staining. Jurkat T cells were left untreated (far left/first panel) or treated for 16 hours under the following conditions: Cells were incubated with 20 ng/mL of recombinant human TRAIL and 10 μg/mL anti-histidine antibody (second panel), or with 20 ng/mL of recombinant human TRAIL and 10 μg/mL anti-histidine antibody preincubated with RIK-2 antibody (third panel), or with 20 ng/mL of recombinant human TRAIL and 10 μg/mL anti-histidine antibody preincubated with mIgG1 antibody (negative control) (far right/fourth panel). Following treatments, cells were evaluated for Annexin-V staining. Cells induced to undergo apoptosis by treatment with recombinant human TRAIL gave a population of cells that was Annexin V-FITC positive (second panel, M2). Annexin V-FITC staining was blocked when cells were incubated with a mixture of recombinant human TRAIL and RIK-2 antibody (third panel, M2). Cells treated with the mixture of recombinant TRAIL and mIgG1 antibody could not block the killing (far right/fourth panel). A small number of Annexin-V positive cells in the untreated population represents a basal level of apoptosis (far left/first panel). The results indicate that clone RIK-2 can block cell mediated killing induced by recombinant human TRAIL as measured by Annexin V-FITC staining of Jurkat cells.
BD Pharmingen™ Purified NA/LE Mouse Anti-Human CD253 - NA/LE - Clone RIK-2 - Isotype Mouse IgG1 - Reactivity Hu - 0.25 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 过滤
- Sterile filtered,0.2 μm filtered
- 内毒素水平
- Endotoxin level is ≤0.01 EU/μg (≤0.001 ng/μg) of protein as determined by the LAL assay.
- 免疫原
- Human TRAIL Transfected Cell Line
- 克隆位点
- RIK
- 亚型
- IgG1
- Top Product
- Discover our top product TNFSF10 Primary Antibody
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anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 39-250) antibody
TNFSF10 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 185-281) antibodyTNFSF10 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 95-281) antibodyTNFSF10 适用: 人 FACS, Func 宿主: 小鼠 Monoclonal 2E5 unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 95-281) antibody (PE)TNFSF10 适用: 人 FACS 宿主: 小鼠 Monoclonal 2E5 PE
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 115-287) antibodyTNFSF10 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 95-281) antibodyTNFSF10 适用: 人 WB, FACS, ICC, IHC (f) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 1-300) antibodyTNFSF10 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) antibodyTNFSF10 适用: 人 WB, ELISA, IHC, IHC (p), Neut 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 1-300) antibodyTNFSF10 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (Internal Region) antibodyTNFSF10 适用: 人 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
- 储存液
- Without preservative
- 注意事项
- This preparation contains no preservatives, thus it should be handled under aseptic conditions.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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: "Involvement of TNF-related apoptosis-inducing ligand in human CD4+ T cell-mediated cytotoxicity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 5, pp. 2639-47, (1999) (PubMed).
: "Activation of apoptosis by Apo-2 ligand is independent of FADD but blocked by CrmA." in: Current biology : CB, Vol. 6, Issue 6, pp. 750-2, (1997) (PubMed).
: "Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors." in: Science (New York, N.Y.), Vol. 277, Issue 5327, pp. 818-21, (1997) (PubMed).
: "Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosis factor cytokine family." in: The Journal of biological chemistry, Vol. 271, Issue 22, pp. 12687-90, (1996) (PubMed).
: "Identification and characterization of a new member of the TNF family that induces apoptosis." in: Immunity, Vol. 3, Issue 6, pp. 673-82, (1996) (PubMed).
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: "Involvement of TNF-related apoptosis-inducing ligand in human CD4+ T cell-mediated cytotoxicity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 5, pp. 2639-47, (1999) (PubMed).
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- 抗原
- TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
- 别名
- CD253 (TNFSF10 产品)
- 别名
- APO2L antibody, Apo-2L antibody, CD253 antibody, TL2 antibody, TRAIL antibody, A330042I21Rik antibody, AI448571 antibody, APO-2L antibody, Ly81 antibody, Trail antibody, tl2 antibody, apo2l antibody, cd253 antibody, trail antibody, apo-2l antibody, trail1 antibody, xtrail1 antibody, tnfsf10l2 antibody, zgc:92320 antibody, TNLG6A antibody, TNF superfamily member 10 antibody, tumor necrosis factor (ligand) superfamily, member 10 antibody, tumor necrosis factor superfamily member 10 antibody, TNF superfamily member 10 S homeolog antibody, TNFSF10 antibody, Tnfsf10 antibody, tnfsf10.S antibody, tnfsf10 antibody
- 背景
- Synonyms: TNFSF10, TRAIL, APO-2L, TL2
- 途径
- Apoptosis, Positive Regulation of Endopeptidase Activity
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