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CCR4 抗体

CCR4 适用: 人 FACS 宿主: 小鼠 Monoclonal 1G1 unconjugated
产品编号 ABIN2689052
发货至: 中国
  • 抗原 See all CCR4 抗体
    CCR4 (Chemokine (C-C Motif) Receptor 4 (CCR4))
    适用
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    宿主
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    小鼠
    克隆类型
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    单克隆
    标记
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    This CCR4 antibody is un-conjugated
    应用范围
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    Flow Cytometry (FACS)
    品牌
    BD Pharmingen™
    产品特性
    The monoclonal antibody 1G1 reacts with CD194, also known as the human CC Chemokine Receptor type 4 (CCR4). CCR4 is expressed on activated Th2 cells, regulatory T cells, activated NK cells, basophils, monocytes and platelets. CCR4 is a seven-transmembrane, G-protein-coupled receptor, and is the specific receptor for CC chemokines, CCL22/MDC/Macrophage-Derived Chemokine and CCL17/TARC/Thymus and Activation-Regulated Chemokine. It has been reported that CCR4 mRNA is expressed mainly in the thymus and spleen. The human CCR4 gene has been mapped to chromosome 3p24. The purified form of this antibody has been reported not to be a neutralizing antibody. The immunogen used to generate the 1G1 hybridoma has been reported to be human CCR4 transfected L1.2 mouse lymphoma cells. Flow cytometric detection of CD194 (CCR4) on human peripheral lymphocytes. Human PBMC were stained with 0.25 μg of the purified Mouse Anti-Human CD194 (CCR4) antibody using a Biotin Goat Anti-Mouse Ig secondary antibody and with PE Streptavidin in conjunction with either a FITC Mouse Anti-Human CD4 antibody (Cat. No. 555346, left panel) or with a FITC Mouse Anti-Human CD8 antibody (Cat. No. 555366, right panel). The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed by using purified mouse IgG1 (Cat. No. 555746) as an isotype control. The quadrant markers for the bivariate dot plots were set based on the isotype control.

    BD Pharmingen™ Purified Mouse Anti-Human CD194 - Purified - Clone 1G1 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg
    纯化方法
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    免疫原
    Human CCR4 Transfected Cell Line
    克隆位点
    1G1
    亚型
    IgG1 kappa
    Top Product
    Discover our top product CCR4 Primary Antibody
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    限制
    仅限研究用
  • 浓度
    0.5 mg/mL
    缓冲液
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store undiluted at 4°C.
  • Campbell, Haraldsen, Pan, Rottman, Qin, Ponath, Andrew, Warnke, Ruffing, Kassam, Wu, Butcher: "The chemokine receptor CCR4 in vascular recognition by cutaneous but not intestinal memory T cells." in: Nature, Vol. 400, Issue 6746, pp. 776-80, (1999) (PubMed).

    DAmbrosio, Iellem, Bonecchi, Mazzeo, Sozzani, Mantovani, Sinigaglia: "Selective up-regulation of chemokine receptors CCR4 and CCR8 upon activation of polarized human type 2 Th cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 10, pp. 5111-5, (1998) (PubMed).

    Sallusto, Lenig, Mackay, Lanzavecchia: "Flexible programs of chemokine receptor expression on human polarized T helper 1 and 2 lymphocytes." in: The Journal of experimental medicine, Vol. 187, Issue 6, pp. 875-83, (1998) (PubMed).

    Imai, Chantry, Raport, Wood, Nishimura, Godiska, Yoshie, Gray: "Macrophage-derived chemokine is a functional ligand for the CC chemokine receptor 4." in: The Journal of biological chemistry, Vol. 273, Issue 3, pp. 1764-8, (1998) (PubMed).

    Samson, Soularue, Vassart, Parmentier: "The genes encoding the human CC-chemokine receptors CC-CKR1 to CC-CKR5 (CMKBR1-CMKBR5) are clustered in the p21.3-p24 region of chromosome 3." in: Genomics, Vol. 36, Issue 3, pp. 522-6, (1997) (PubMed).

    Imai, Baba, Nishimura, Kakizaki, Takagi, Yoshie: "The T cell-directed CC chemokine TARC is a highly specific biological ligand for CC chemokine receptor 4." in: The Journal of biological chemistry, Vol. 272, Issue 23, pp. 15036-42, (1997) (PubMed).

    Power, Meyer, Nemeth, Bacon, Hoogewerf, Proudfoot, Wells: "Molecular cloning and functional expression of a novel CC chemokine receptor cDNA from a human basophilic cell line." in: The Journal of biological chemistry, Vol. 270, Issue 33, pp. 19495-500, (1995) (PubMed).

  • 抗原
    CCR4 (Chemokine (C-C Motif) Receptor 4 (CCR4))
    别名
    CD194 (CCR4 产品)
    别名
    CC-CKR-4 antibody, CD194 antibody, CKR4 antibody, CMKBR4 antibody, ChemR13 antibody, HGCN:14099 antibody, K5-5 antibody, Ccr4 antibody, C-C CKR-4 antibody, CHEMR1 antibody, Cmkbr4 antibody, LESTR antibody, Sdf1r antibody, C-C motif chemokine receptor 4 antibody, chemokine (C-C motif) receptor 4 antibody, CCR4 antibody, Ccr4 antibody
    背景
    Synonyms: CCR4, C-C chemokine receptor type 4, CMKBR4, K5-5
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