我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

CD19 抗体 (Biotin)

Name: CD19 抗体 (Biotin)
SKU: ABIN2689050
Availability: OutOfStock

CD19 适用: 小鼠 FACS, IF 宿主: 大鼠 Monoclonal 1D3 Biotin

(12 references)

产品编号 ABIN2689050

发货至: 中国

Datasheet as PDF

抗原 See all CD19 抗体

CD19 (CD19 Molecule (CD19))
适用
293

162

48

30

19

10

7

6

3

3

2

2

2

2

2

2

2

2
小鼠
宿主
172

140

47

4
大鼠
Compatible Secondaries
克隆类型
231

128

2
单克隆
标记
112

22

21

19

16

8

8

8

7

7

7

7

7

7

7

6

6

6

4

4

4

4

4

4

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1
This CD19 antibody is conjugated to Biotin
应用范围
263

116

72

62

60

39

23

20

17

14

14

9

5

4

3

3

2

2

2

1

1

1

1

1

1

1
Flow Cytometry (FACS), Immunofluorescence (IF)

品牌

BD Pharmingen™

产品特性

The 1D3 antibody reacts with CD19, a B lymphocyte-lineage differentiation antigen. CD19, a 95 kDa transmembrance glycoprotein, is a member of the immunoglobulin superfamily and is expressed throughout B-lymphocyte development from the pro-B cell through the mature B-cell stages. Terminally differentiated plasma cells do not express CD19. On the surface of mature B cells, the CD19 Molecule associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to promote cellular activation. Studies with CD19-deficient mice have suggested that the level of CD19 expression affects the generation and maturation of B cells in the bone marrow and periphery. B-1 lineage B cells, also known as CD5+ B cells, are drastically reduced or absent in CD19-deficient mice. Increased levels of CD19 expression correlate with increased frequencies of peritonal and splenic B-1 cells and reduced numbers of conventional B lymphocytes in the periphery. CD19 participates in B-lymphocyte development, B-cell activation, maturation of memory B cells and regulation of tolerance. CD19 has also been detected on peritoneal mast cells, co-localized with CD21/CD35, and it is proposed to play a role in complement-mediated mast-cell activation. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two-color analysis of the expression of CD19 on mouse spleen B cells. BALB/c splenocytes were stained with PE-conjugated anti-mouse CD3e mAb 145-2C11 (Cat. No. 553063) in the absence (left panel) or presence (right panel) of biotin-conjugated 1D3 mAb followed by Avidin-FITC (Cat. No. 554057). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Biotin Rat Anti-Mouse CD19 - Biotin - Clone 1D3 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Mouse CD19 Transfected Cell Line

克隆位点

1D3

亚型

IgG2a kappa

Top Product

Discover our top product CD19 Primary Antibody
anti-CD19 Molecule (CD19) (AA 485-556) antibody
CD19 适用: 人, 小鼠, 大鼠, 绵羊 FACS, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-CD19 Molecule (CD19) (AA 151-250) antibody
CD19 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-CD19 Molecule (CD19) (AA 20-291) antibody
CD19 适用: 人 FACS, WB, IF, IHC, ELISA 宿主: 小鼠 Monoclonal 8G11B7 unconjugated

anti-CD19 Molecule (CD19) (C-Term) antibody
CD19 适用: 人, 小鼠, 猴, 大鼠, 绵羊, Pig, Cow, 豚鼠, 犬, 驴, 山羊, Hamster, 马, 兔, Avian, Cat, 小鸡 WB 宿主: 山羊 Polyclonal unconjugated

anti-CD19 Molecule (CD19) (pTyr531) antibody
CD19 适用: 人, 小鼠 FACS, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-CD19 Molecule (CD19) (AA 96-281) antibody
CD19 适用: 人 FACS, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal CD19-3116 unconjugated

anti-CD19 Molecule (CD19) antibody
CD19 适用: 人 FACS, WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-CD19 Molecule (CD19) (AA 143-172) antibody
CD19 适用: 人 FACS, WB, IF, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-CD19 Molecule (CD19) (AA 160-258) antibody
CD19 适用: 人 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-CD19 Molecule (CD19) (AA 20-291) antibody
CD19 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4C6G1 unconjugated

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
浓度

0.5 mg/mL

缓冲液

Aqueous buffered solution containing ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

储存条件

4 °C

储存方法

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Gommerman, Oh, Zhou, Tedder, Maurer, Galli, Carroll: "A role for CD21/CD35 and CD19 in responses to acute septic peritonitis: a potential mechanism for mast cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 12, pp. 6915-21, (2000) (PubMed).

Sato, Jansen, Tedder: "CD19 and CD22 expression reciprocally regulates tyrosine phosphorylation of Vav protein during B lymphocyte signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 24, pp. 13158-62, (1998) (PubMed).

Inaoki, Sato, Weintraub, Goodnow, Tedder: "CD19-regulated signaling thresholds control peripheral tolerance and autoantibody production in B lymphocytes." in: The Journal of experimental medicine, Vol. 186, Issue 11, pp. 1923-31, (1997) (PubMed).

Sato, Miller, Howard, Tedder: "Regulation of B lymphocyte development and activation by the CD19/CD21/CD81/Leu 13 complex requires the cytoplasmic domain of CD19." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 7, pp. 3278-87, (1997) (PubMed).

Sato, Steeber, Jansen, Tedder: "CD19 expression levels regulate B lymphocyte development: human CD19 restores normal function in mice lacking endogenous CD19." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 10, pp. 4662-9, (1997) (PubMed).

Krop, Shaffer, Fearon, Schlissel: "The signaling activity of murine CD19 is regulated during cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 1, pp. 48-56, (1996) (PubMed).

Krop, de Fougerolles, Hardy, Allison, Schlissel, Fearon: "Self-renewal of B-1 lymphocytes is dependent on CD19." in: European journal of immunology, Vol. 26, Issue 1, pp. 238-42, (1996) (PubMed).

Sato, Ono, Steeber, Pisetsky, Tedder: "CD19 regulates B lymphocyte signaling thresholds critical for the development of B-1 lineage cells and autoimmunity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 10, pp. 4371-8, (1996) (PubMed).

Engel, Zhou, Ord, Sato, Koller, Tedder: "Abnormal B lymphocyte development, activation, and differentiation in mice that lack or overexpress the CD19 signal transduction molecule." in: Immunity, Vol. 3, Issue 1, pp. 39-50, (1995) (PubMed).

Rickert, Rajewsky, Roes: "Impairment of T-cell-dependent B-cell responses and B-1 cell development in CD19-deficient mice." in: Nature, Vol. 376, Issue 6538, pp. 352-5, (1995) (PubMed).

Tedder, Zhou, Engel: "The CD19/CD21 signal transduction complex of B lymphocytes." in: Immunology today, Vol. 15, Issue 9, pp. 437-42, (1994) (PubMed).

Fearon: "The CD19-CR2-TAPA-1 complex, CD45 and signaling by the antigen receptor of B lymphocytes." in: Current opinion in immunology, Vol. 5, Issue 3, pp. 341-8, (1993) (PubMed).
抗原

CD19 (CD19 Molecule (CD19))

别名

CD19 (CD19 产品)

别名

B4 antibody, CVID3 antibody, AW495831 antibody, CD19 antibody, CD19 molecule antibody, CD19 antigen antibody, CD19 antibody, Cd19 antibody

途径

Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway