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HSPD1 抗体

Name: HSPD1 抗体
SKU: ABIN263948
Availability: OutOfStock

HSPD1 适用: 人, 小鼠, 大鼠, 小鸡, 猴, 犬, Hamster, Cow, 兔, Pig, 豚鼠, Bacteria, Saccharomyces cerevisiae, 植物, Whitefly WB, FACS, IHC (fro) 宿主: 小鼠 Monoclonal LK2 unconjugated

Hsp60 visualized using AM03145PU (Clone LK2), tested on Human colon cancer cells.

IHC

Immunohistochemistry analysis of Listeria infected mice spleens 6 days after infection with L-monocytogenes using AM03145PU (Clone LK2).

IHC (fro)

3 images

(7 references)

产品编号 ABIN263948

发货至: 中国

Datasheet as PDF

抗原 See all HSPD1 抗体

HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))
适用
209

124

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74

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64

62

61

60

46

41

36

26

25

21

17

17

14

14

13

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10

10

10

10

4

4

4

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4

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3

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2

1

1

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1

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1
人, 小鼠, 大鼠, 小鸡, 猴, 犬, Hamster, Cow, 兔, Pig, 豚鼠, Bacteria, Saccharomyces cerevisiae, 植物, Whitefly
宿主
123

114

3
小鼠
Compatible Secondaries
克隆类型
124

114
单克隆
标记
113

19

10

7

7

7

7

6

6

6

5

5

5

5

5

5

4

3

3

2

2

2

2

2

2
This HSPD1 antibody is un-conjugated
应用范围
189

105

99

92

67

65

27

27

25

24

12

5

5

4

3

3

2

2

1

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

特异性

Recognizes amino acids 383-447. Recognizes a ~60 kDa protein, corresponding to the Molecular Mass of HSP60 on SDS-PAGE immunoblots.

产品特性

Synonyms: HSP-60, HSPD1, Heat shock protein 60, Chaperonin 60, HuCHA60, GROEL, GroEL Homolog,CPN60, 60 kDa heat shock protein mitochondrial, 60 kDa chaperonin, Mitochondrialmatrix protein P1, P60 lymphocyte protein

纯化方法

Purified

免疫原

Human Hsp60 produced through recombinant DNA methods in E. coli

克隆位点

LK2

亚型

IgG1

Top Product

Discover our top product HSPD1 Primary Antibody
anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) (AA 501-573) antibody
HSPD1 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibody
HSPD1 适用: 人 WB, IHC, FACS, ELISA, IP 宿主: 小鼠 Monoclonal LK1 unconjugated

anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibody
HSPD1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 3G8 unconjugated

anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibody
HSPD1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IEM 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) (AA 172-343) antibody
HSPD1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) (AA 1-573) antibody
HSPD1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) (AA 316-573) antibody
HSPD1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) (AA 1-573) antibody
HSPD1 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibody
HSPD1 适用: 人, 小鼠, 大鼠, 小鸡, 猴, 犬, Hamster, Cow, 兔, Pig, 绵羊, Drosophila melanogaster, 非洲爪蟾 WB, IHC, FACS, IF, ISt, StM 宿主: 小鼠 Monoclonal LK1 unconjugated

anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) (AA 340-374) antibody
HSPD1 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB55969 unconjugated

应用备注

Western blot (10): 0.25 μg/mL. Immunohistochemistry on Frozen Sections. This Clone is reported to work on Paraffin Sections. Flow Cytometry (12).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

1.0 mg/mL

缓冲液

PBS containing 0.1 mM PMSF and 50 % Glycerol.

储存条件

4 °C/-20 °C

储存方法

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

有效期

12 months
Britti, Delaspre, Feldman, Osborne, Greif, Tamarit, Ros: "Frataxin-deficient neurons and mice models of Friedreich ataxia are improved by TAT-MTScs-FXN treatment." in: Journal of cellular and molecular medicine, Vol. 22, Issue 2, pp. 834-848, (2019) (PubMed).

Teigen, Orczewska, McLaughlin, OBrien: "Cold acclimation increases levels of some heat shock protein and sirtuin isoforms in threespine stickleback." in: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, Vol. 188, pp. 139-47, (2015) (PubMed).

Kötter, Unger, Hamdani, Lang, Vorgerd, Nagel-Steger, Linke: "Human myocytes are protected from titin aggregation-induced stiffening by small heat shock proteins." in: The Journal of cell biology, Vol. 204, Issue 2, pp. 187-202, (2014) (PubMed).

Surazynski, Miltyk, Prokop, Palka: "The effect of estrogen on prolidase-dependent regulation of HIF-1? expression in breast cancer cells." in: Molecular and cellular biochemistry, Vol. 379, Issue 1-2, pp. 29-36, (2013) (PubMed).

van der Lubbe, Lim, Meima, van Veghel, Rosenbaek, Mutig, Danser, Fenton, Zietse, Hoorn: "Aldosterone does not require angiotensin II to activate NCC through a WNK4-SPAK-dependent pathway." in: Pflügers Archiv : European journal of physiology, Vol. 463, Issue 6, pp. 853-63, (2012) (PubMed).

Hung, Li, Lee, Chang, Chien, Lee: "Green tea extract supplementation ameliorates CCl4-induced hepatic oxidative stress, fibrosis, and acute-phase protein expression in rat." in: Journal of the Formosan Medical Association = Taiwan yi zhi, Vol. 111, Issue 10, pp. 550-9, (2012) (PubMed).

Gao, Brosnan, Raine: "Experimental autoimmune encephalomyelitis. Qualitative and semiquantitative differences in heat shock protein 60 expression in the central nervous system." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 7, pp. 3548-56, (1995) (PubMed).
抗原

HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

别名

HSP60 (HSPD1 产品)

别名

CPN60 antibody, GROEL antibody, HLD4 antibody, HSP-60 antibody, HSP60 antibody, HSP65 antibody, HuCHA60 antibody, SPG13 antibody, chaperonin antibody, cpn60 antibody, groel antibody, hld4 antibody, hsp60 antibody, hsp65 antibody, spg13 antibody, 60kDa antibody, Hsp60 antibody, 12 antibody, BP5 antibody, CG12101 antibody, Cpn60 antibody, Dmel\\CG12101 antibody, Dmhsp60 antibody, G62 antibody, HSP60A antibody, Hsp60A antibody, IEF16 antibody, Mmp-P1 antibody, SSP 7506 antibody, d-hsp60 antibody, hsp60A antibody, l(1)10Ac antibody, l(1)BP5 antibody, l(1)G8 antibody, l(1)HM21 antibody, l(1)L12 antibody, l(1)dp025 antibody, cb863 antibody, fa04a05 antibody, fb22d10 antibody, fi27b05 antibody, id:ibd2197 antibody, sb:cb144 antibody, wu:fa04a05 antibody, wu:fb22d10 antibody, wu:fi04a12 antibody, wu:fi27b05 antibody, MIF4 antibody, MNA2 antibody, mopA antibody, groL antibody, crpA antibody, Hspd1-30p antibody, heat shock protein family D (Hsp60) member 1 antibody, heat shock protein family D (Hsp60) member 1 S homeolog antibody, 60 kDa heat shock protein, mitochondrial antibody, heat shock protein 1 (chaperonin) antibody, Heat shock protein 60A antibody, heat shock 60 protein 1 antibody, chaperone ATPase HSP60 antibody, molecular chaperone GroEL antibody, thermosome subunit antibody, chaperonin GroEL antibody, mitochondrial chaperonin antibody, heat shock protein family D member 1 antibody, HSPD1 antibody, hspd1.S antibody, hspd1 antibody, LOC100414401 antibody, Hspd1 antibody, Hsp60A antibody, HSP60 antibody, groEL antibody, MMP_RS07785 antibody, groEl antibody, LOC100136430 antibody

背景

In both prokaryotic and eukarytic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. Hsp60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian Hsp60 (1-3). Whereas mammalian Hsp60 is localized within the mitochondria, plant Hsp60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that Hsp60 is present in so many different species. The common characteristics of the Hsp60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (4). These similarities are supported by recent studies where the single-ring human mitochondrial homolog, Hsp60 with its co-chaperonin, Hsp10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of Hsp60-Hsp10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES (5). Another important function of Hsp60 and Hsp10 is their protective functions against infection and cellular stress. Hsp60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes (6-9).Synonyms: 60 kDa chaperonin, 60 kDa heat shock protein mitochondrial, CPN60, Chaperonin 60, GROEL, GroEL Homolog, HSP-60, HSPD1, Heat shock protein 60, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein

基因ID

3329

UniProt

P10809

途径

Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity