DMPO 抗体 (Atto 594)
-
- 抗原
- DMPO
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- Atto 594
-
应用范围
- Immunoprecipitation (IP), ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Antibody Array (AA)
- 特异性
- Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA.
- 纯化方法
- Protein G Purified
- 免疫原
- 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin
- 克隆位点
- N1664A
- 亚型
- IgG1
-
anti-DMPO antibody (FITC)
IP, ELISA, WB, ICC, IF, IHC, AA 宿主: 小鼠 Monoclonal N1664A FITC
anti-DMPO antibodyIP, ELISA, WB, ICC, IF, IHC, AA 宿主: 小鼠 Monoclonal N1664A unconjugated
anti-DMPO antibody (Biotin)IP, ELISA, WB, ICC, IF, IHC, AA 宿主: 小鼠 Monoclonal N1664A Biotin
anti-DMPO antibody (HRP)IP, ELISA, WB, ICC, IF, IHC, AA 宿主: 小鼠 Monoclonal N1664A HRP
anti-DMPO antibody (Alkaline Phosphatase (AP))IP, ELISA, WB, ICC, IF, IHC, AA 宿主: 小鼠 Monoclonal N1664A Alkaline Phosphatase (AP)
anti-DMPO antibody (PE)IP, ELISA, WB, ICC, IF, IHC, AA 宿主: 小鼠 Monoclonal N1664A PE
anti-DMPO antibody (APC)IP, ELISA, WB, ICC, IF, IHC, AA 宿主: 小鼠 Monoclonal N1664A APC
anti-DMPO antibody (PerCP)IP, ELISA, WB, ICC, IF, IHC, AA 宿主: 小鼠 Monoclonal N1664A PerCP
anti-DMPO antibody (Atto 488)IP, ELISA, WB, ICC, IF, IHC, AA 宿主: 小鼠 Monoclonal N1664A Atto 488
anti-DMPO antibody (Atto 390)IP, ELISA, WB, ICC, IF, IHC, AA 宿主: 小鼠 Monoclonal N1664A Atto 390
-
- 应用备注
-
- WB (1:1000)
- ICC/IF (1:100)
- ELISA (1:100)
- IP (25 μg)
- optimal dilutions for assays should be determined by the user.
- 说明
-
A 1:1000 dilution of ABIN2482172 was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100 ng/lane by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Conjugated antibodies should be stored at 4°C
-
- 抗原
- DMPO
- 物质类
- Chemical
- 背景
- The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states (1). The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic to cells and animals, and possesses convenient pharmacokinetics (uptake, distribution, metabolism and excretion) in biological systems (2-6). Recent studies have determined that nitric oxide may substantially affect the quantitative determination of DMPO adducts, and therefore extra caution is required when studying generation of these species in the presence of nitric oxide or its radicals (1). DMPO adducts can be generated with protein and DNA radicals (7).
-
