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Arylsulfatase A 抗体 (Internal Region)

Verified ARSA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, FACS, IF 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN238536
发货至: 中国
  • 抗原 See all Arylsulfatase A (ARSA) 抗体
    Arylsulfatase A (ARSA)
    抗原表位
    • 19
    • 15
    • 6
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    适用
    • 56
    • 35
    • 33
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    人, 小鼠, 大鼠
    宿主
    • 55
    • 16
    山羊
    克隆类型
    • 57
    • 14
    多克隆
    标记
    • 35
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Arylsulfatase A antibody is un-conjugated
    应用范围
    • 54
    • 21
    • 13
    • 13
    • 13
    • 9
    • 8
    • 7
    • 5
    • 4
    • 4
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
    原理
    Arylsulfatase A
    序列
    YDLSKDPGEN YN
    交叉反应
    人, 小鼠, 大鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-YDLSKDPGENYN, from the internal region of the protein sequence according to NP_000478.2.
    亚型
    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Brain (Cortex) and Testis. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 60-65Da band observed in Mouse and Rat Testis lysates (calculated MW of 53.7 kDa according to Mouse NP_033843.2 and Rat NP_001030105.2,). This molecular weight is routinely observed by other sources and was successfully blocked by inc

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the Golgi apparatus of HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10u

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Kanbe, Kamijo, Nakajima, Tanaka, Sugiyama, Wang, Fang, Hara, Gonzalez, Aoyama: "Chronic ethanol consumption decreases serum sulfatide levels by suppressing hepatic cerebroside sulfotransferase expression in mice." in: Archives of toxicology, Vol. 88, Issue 2, pp. 367-79, (2014) (PubMed).

    Kimura, Nakajima, Kamijo, Tanaka, Wang, Hara, Sugiyama, Tanaka, Gonzalez, Aoyama: "Hepatic Cerebroside Sulfotransferase Is Induced by PPAR? Activation in Mice." in: PPAR research, Vol. 2012, pp. 174932, (2012) (PubMed).

    Kiebish, Young, Lehman, Han: "Chronic caloric restriction attenuates a loss of sulfatide content in PGC-1?-/- mouse cortex: a potential lipidomic role of PGC-1? in neurodegeneration." in: Journal of lipid research, Vol. 53, Issue 2, pp. 273-81, (2012) (PubMed).

    Zhang, Nakajima, Kamijo, Li, Hu, Kannagi, Kyogashima, Aoyama, Hara: "Acute kidney injury induced by protein-overload nephropathy down-regulates gene expression of hepatic cerebroside sulfotransferase in mice, resulting in reduction of liver and serum sulfatides." in: Biochemical and biophysical research communications, Vol. 390, Issue 4, pp. 1382-8, (2009) (PubMed).

  • 抗原
    Arylsulfatase A (ARSA)
    别名
    ARSA (ARSA 产品)
    别名
    ARSA antibody, zgc:101575 antibody, arsa antibody, AS-A antibody, ASA antibody, AW212749 antibody, As-2 antibody, As2 antibody, TISP73 antibody, MLD antibody, mld antibody, arylsulfatase A antibody, arylsulfatase antibody, arylsulfatase A, gene 1 S homeolog antibody, ARSA antibody, arsa antibody, arsA antibody, RB6599 antibody, Arsa antibody, arsa.1.S antibody
    背景
    ARSA, arylsulfatase A, MLD, cerebroside-sulfatase, cerebroside 3-sulfatase
    基因ID
    410, 11883, 315222
    NCBI登录号
    NP_000478
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