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- 抗原 See all CD300a (CD300A) 抗体
- CD300a (CD300A)
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD300a antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS)
- 特异性
- This antibody reacts with CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25 % of circulating T cells and 15 % of circulating B cells.
- 交叉反应 （详细）
- Species reactivity (tested):Human
- 产品特性
- Synonyms: CLM-8, CMRF35-H, CMRF35-H9, CMRF-35-H9, IRC1/IRC2, IRp60, CMRF35-like molecule 8,Inhibitory receptor protein 60, NK inhibitory receptor, CD300 antigen-like family member A
- 纯化方法
- Protein A affinity chromatography.
- 免疫原
- HPB human acute lymphoid leukemia cell line.
- 克隆位点
- MEM-260
- 亚型
- IgG1
- Top Product
- Discover our top product CD300A Primary Antibody
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anti-CD300a (CD300A) (AA 18-180) antibody
CD300A 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 7H8E4 unconjugated
anti-CD300a (CD300A) antibody (PE)CD300A 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal MEM-260 PE
anti-CD300a (CD300A) antibodyCD300A 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal MEM-260 unconjugated
anti-CD300a (CD300A) (AA 98-149) antibodyCD300A 适用: 人 IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD300a (CD300A) (AA 1-299) antibodyCD300A 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-CD300a (CD300A) (Internal Region) antibodyCD300A 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD300a (CD300A) antibody (APC)CD300A 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-260 APC
anti-CD300a (CD300A) antibody (PE)CD300A 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-260 PE
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- 应用备注
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Flow Cytometry. Western Blotting (Non-reducing conditions): Positive material: HPB cell lineNegative material: JURKAT human cell line, RAJI human cell line. Sample preparation: Resuspend approx.50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysatewith non-reducing SDS-PAGE sample buffer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.4 containing 15 mM sodium azide as preservative
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- CD300a (CD300A)
- 别名
- CD300A / CMRF35H (CD300A 产品)
- 别名
- CD300L-B1 antibody, CD300A antibody, clm8 antibody, B230315M08Rik antibody, Clm8 antibody, LMIR1 antibody, MAIR-I antibody, MAIR-Ia antibody, MMAC8 antibody, Pigr4 antibody, mcpir1 antibody, CLM-8 antibody, CMRF-35-H9 antibody, CMRF-35H antibody, CMRF35-H antibody, CMRF35-H9 antibody, CMRF35H antibody, CMRF35H9 antibody, IGSF12 antibody, IRC1 antibody, IRC1/IRC2 antibody, IRC2 antibody, IRp60 antibody, CD300 molecule like family member g antibody, CD300a molecule antibody, CMRF35-like molecule 8 antibody, CD300A molecule antibody, Cd300a molecule antibody, CD300LG antibody, CD300A antibody, LOC483289 antibody, clm8 antibody, Cd300a antibody
- 背景
- CD300a (CMRF-35H, IRp60) is a non-MHC-specific inhibitory receptor of immunoglobulin superfamily, which contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that associate with SH2-containing phosphatases SHP-1 and SHP-2. CD300a is expressed on many cell types including T cells, NK cells, neutrophils, eosinophils or mast cells. Its triggering inhibits activating signals such as those of IL5, GM-CSF or eotaxin, as well as supresses mast cell degranulation or NK cell cytotoxic activity.Synonyms: CD300 antigen-like family member A, CLM-8, CMRF-35-H9, CMRF35-H, CMRF35-H9, CMRF35-like molecule 8, IRC1/IRC2, IRp60, Inhibitory receptor protein 60, NK inhibitory receptor
- 基因ID
- 11314
- UniProt
- Q9UGN4
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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