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RFP 抗体 (Red Fluorescent Protein)

Details for Product anti-RFP Antibody No. ABIN233863, 供应商: Log in to see
抗原
适用
Discosoma
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21
10
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2
1
1
宿主

69
37
14
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1
克隆类型
多克隆
标记
This RFP antibody is un-conjugated
7
5
5
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
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1
1
应用范围
Western Blotting (WB)
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71
47
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29
7
5
4
3
2
1
1
1
1
1
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Supplier
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免疫原 Recombinant RFP expressed from E. coli
亚型 IgG
纯化方法 Affinity purified
别名 RFP
背景 Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. RFP is the recent discovered protein that has the similar function and applications as GFP. Either GFP or RFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP or RFP can fuse with proteins of interest without interfering significantly with their assembly and function.
Synonyms: Red Fluorescent Protein
基因ID 3229046
UniProt P00273
研究领域 Tags/Labels
应用备注 Western blotting (0.5-4 μg/mL). Based on researchers' feedback, it can also be used in immunoprecipitation (10-20 μg/mL) and immunofluorescence. However, the optimal conditions should be determined individually.
限制 仅限研究用
状态 Liquid
浓度 0.5 mg/mL
缓冲液 100 μg (0.5 mg/mL) affinity purified rabbit anti-RFP polyclonall antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 5 mM EDTA and 0.03 % proclin
储存液 ProClin
注意事项 This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 The antibody solution should be gently mixed before use.
储存条件 -20 °C
储存方法 For long-term storage, aliquot and freeze at -70 °C.
有效期 12 months
厂商提供的图像
Western Blotting (WB) image for anti-RFP 抗体 (Red Fluorescent Protein) (ABIN233863) Western blot analysis with recombinant RFP. Lane 1: 10 ng Lane 2: 50 ng Lane 3: 250 n...
有引用在: Manzo, Silies, Gohl, Scott: "Motor neurons controlling fluid ingestion in Drosophila." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 16, pp. 6307-12, 2012 (PubMed).