GFP 抗体 (Green Fluorescent Protein)

Details for Product anti-GFP Antibody No. ABIN223515, 供应商: Log in to see
抗原
  • green fluorescent protein
  • gfp
适用
Aequorea victoria
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16
5
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1
1
宿主

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16
9
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2
克隆类型
多克隆
标记
This GFP antibody is un-conjugated
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28
14
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2
2
2
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1
1
1
1
1
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应用范围
Immunoprecipitation (IP), Western Blotting (WB)
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8
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3
2
2
1
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选项
Supplier
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Supplier Product No.
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免疫原 Recombinant EGFP
亚型 IgG
特异性 The antibody reacts with GFP, and its variants EGFP, RFP, YFP, and CFP, etc.
纯化方法 Affinity purified
别名 GFP
背景 Green fluorescent protein (GFP) is a spontaneously fluorescent protein isolated from pacific jellyfish, Aequorea victoria. It transduces the blue chemiluminescence into green fluorescent light. Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. GFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP can fuse with proteins of interest without interfering significantly with their assembly and function.
Synonyms: Green Fluorescent Protein
基因ID 110612126
研究领域 Tags/Labels
应用备注 Western blotting (0.5-4 μg/mL) and in immunoprecipitation (10-20 μg/mL). However, the optimal conditions should be determined individually. The antibody reacts with GFP, and its variants EGFP, RFP, YFP, and CFP, etc. Recombinant EGFP, RFP, YFP and CFP can be used as positive controls.
限制 仅限研究用
状态 Liquid
浓度 0.2 mg/mL
缓冲液 100 μg (0.2 mg/mL) affinity purified rabbit anti-GFP polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.
储存液 Thimerosal (Merthiolate)
注意事项 This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 The antibody solution should be gently mixed before use.
储存条件 -20 °C
储存方法 For long-term storage, aliquot and freeze at -70 °C.
有效期 12 months
厂商提供的图像
Western Blotting (WB) image for anti-Green Fluorescent Protein (GFP) antibody (ABIN223515) Western blot analysis of GFP antibody using recombinant RFP (dsRed, Lane 1 & 2), YFP ...
有引用在: Maus, Jairaman, Stathopulos, Muik, Fahrner, Weidinger, Benson, Fuchs, Ehl, Romanin, Ikura, Prakriya, Feske: "Missense mutation in immunodeficient patients shows the multifunctional roles of coiled-coil domain 3 (CC3) in STIM1 activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, Issue 19, pp. 6206-11, 2015 (PubMed).

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Hsu, Lin, Chang: "Synergetic regulation of translational reading-frame switch by ligand-responsive RNAs in mammalian cells." in: Nucleic acids research, Vol. 42, Issue 22, pp. 14070-82, 2014 (PubMed).

Martínez-Fábregas, Díaz-Moreno, González-Arzola, Janocha, Navarro, Hervás, Bernhardt, Velázquez-Campoy, Díaz-Quintana, De la Rosa: "Structural and functional analysis of novel human cytochrome C targets in apoptosis." in: Molecular & cellular proteomics : MCP, Vol. 13, Issue 6, pp. 1439-56, 2014 (PubMed).

Mellergaard, Skovbakke, Schneider, Lauridsen, Andresen, Jensen, Skov: "N-glycosylation of asparagine 8 regulates surface expression of major histocompatibility complex class I chain-related protein A (MICA) alleles dependent on threonine 24." in: The Journal of biological chemistry, Vol. 289, Issue 29, pp. 20078-91, 2014 (PubMed).

Martínez-Fábregas, Díaz-Moreno, González-Arzola, Janocha, Navarro, Hervás, Bernhardt, Díaz-Quintana, De la Rosa: "New Arabidopsis thaliana cytochrome c partners: a look into the elusive role of cytochrome c in programmed cell death in plants." in: Molecular & cellular proteomics : MCP, Vol. 12, Issue 12, pp. 3666-76, 2013 (PubMed).

Makovski, Yaffe, Shpungin, Nir: "Intronic promoter drives the BORIS-regulated expression of FerT in colon carcinoma cells." in: The Journal of biological chemistry, Vol. 287, Issue 9, pp. 6100-12, 2012 (PubMed).

Andresen, Skovbakke, Persson, Hagemann-Jensen, Hansen, Jensen, Skov: "2-deoxy D-glucose prevents cell surface expression of NKG2D ligands through inhibition of N-linked glycosylation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 4, pp. 1847-55, 2012 (PubMed).

Bhat, Folimonova, Cole, Ballard, Lei, Watson, Sumner, Nelson: "Influence of host chloroplast proteins on Tobacco mosaic virus accumulation and intercellular movement." in: Plant physiology, Vol. 161, Issue 1, pp. 134-47, 2012 (PubMed).

Welman, Serrels, Brunton, Ditzel, Frame: "Two-color photoactivatable probe for selective tracking of proteins and cells." in: The Journal of biological chemistry, Vol. 285, Issue 15, pp. 11607-16, 2010 (PubMed).

Kock, Bulgakova, Knust, Sinning, Panneels: "Targeting of Drosophila rhodopsin requires helix 8 but not the distal C-terminus." in: PLoS ONE, Vol. 4, Issue 7, pp. e6101, 2009 (PubMed).

Creighton, Zhu, Alexeyev, Stevens: "Spectrin-anchored phosphodiesterase 4D4 restricts cAMP from disrupting microtubules and inducing endothelial cell gap formation." in: Journal of cell science, Vol. 121, Issue Pt 1, pp. 110-9, 2007 (PubMed).

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