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His 标记 抗体

ABIN223514 产品详细信息, 供应商: Log in to see
抗原
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
适用
标记
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宿主

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克隆类型
多克隆
标记
非结合性
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应用范围
Immunochromatography (IC), Immunoprecipitation (IP), Western Blotting (WB)
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Supplier
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免疫原 6xHis synthetic peptide
Blocking Peptide Blocking peptide for this product available: ABIN412778
序列 HHHHHH
亚型 IgG
特异性 The antibody detects His-tagged recombinant proteins.
纯化方法 Affinity purified
别名 His-Tag
物质类 Tag
背景 A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.
Synonyms: His tag, HHHHHH tag
研究领域 Tags/Labels
应用备注 Western blotting (1 μg/mL), immunoprecipitation (40 μg/mL), and immunocytochemistry (2 μg/mL). However, the optimal conditions should be determined individually. The antibody detects His-tagged recombinant proteins.
限制 仅限研究用
状态 Liquid
浓度 0.5 mg/mL
缓冲液 100 μg (0.5 mg/mL) peptide affinity purified rabbit anti-His-Tag polyclonal antibody in phosphate buffered saline (PBS, pH 7.2), containing 50 % glycerol, 1 % BSA, 0.02 % thimerosal.
储存液 Thimerosal (Merthiolate)
注意事项 This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 The antibody solution should be gently mixed before use.
储存条件 -20 °C
储存方法 For long-term storage, aliquot and freeze at -70 °C.
有效期 12 months
厂商提供的图像
Western Blotting (WB) image for anti-His 标记 抗体 (ABIN223514) anti-His Tag antibody
有引用在: Yin, Johns, Lawrence, Xu, Reddi, Bishop, Varner, Fuster: "Lymphatic endothelial heparan sulfate deficiency results in altered growth responses to vascular endothelial growth factor-C (VEGF-C)." in: The Journal of biological chemistry, Vol. 286, Issue 17, pp. 14952-62, 2011 (PubMed).

Tsang, Felek, Krukonis: "Ail binding to fibronectin facilitates Yersinia pestis binding to host cells and Yop delivery." in: Infection and immunity, Vol. 78, Issue 8, pp. 3358-68, 2010 (PubMed).