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Myc 标记 抗体

ABIN223509 产品详细信息, 供应商: Log in to see
抗原
适用
标记
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宿主

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克隆类型
多克隆
标记
非结合性
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应用范围
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
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免疫原 Synthetic peptide corresponding to residues surrounding amino acid 413 of human c-Myc
Blocking Peptide Blocking peptide for this product available: ABIN412841
序列 EQKLISEEDL
亚型 IgG
特异性 The antibody detects Myc-Tag proteins overexpressed in cells.
It does not detect endogenous levels of the transcription factor c-Myc.
纯化方法 Affinity purified
别名 C-Myc Epitope Tag
物质类 Tag
背景 Epitope tags are usually for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian systems.
Synonyms: MYC , C-MYC , c-Myc
基因ID 4609
UniProt P01106
研究领域 Transcription Factors
应用备注 Western blotting (1-2 μg/mL), immunoprecipitation (20-40 μg/mL), and immunocytochemistry (10-20 μg/mL). However, the optimal conditions should be determined individually. The antibody detects Myc-Tag proteins overexpressed in cells. It does not detect endogenous levels of the transcription factor c-Myc.
限制 仅限研究用
状态 Liquid
浓度 0.5 mg/mL
缓冲液 100 μg (0.5 mg/mL) peptide affinity purified rabbit anti-Myc-Tag polyclonal antibody in phosphate buffered saline (PBS, pH 7.2), containing 50 % glycerol, 1 % BSA, 0.02 % thimerosal.
储存液 Thimerosal (Merthiolate)
注意事项 This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 The antibody solution should be gently mixed before use.
储存条件 -20 °C
储存方法 For long-term storage, aliquot and freeze at -70 °C.
有效期 12 months
厂商提供的图像
 image for anti-Myc 标记 抗体 (ABIN223509) anti-Myc Tag antibody
有引用在: Kumar, Shum, Glaunsinger: "Importin alpha-mediated nuclear import of cytoplasmic poly(A) binding protein occurs as a direct consequence of cytoplasmic mRNA depletion." in: Molecular and cellular biology, Vol. 31, Issue 15, pp. 3113-25, 2011 (PubMed).

Lei, Shi, Basu, Huq, Routhier, Day, Jin: "Proteolytic processing of angiopoietin-like protein 4 by proprotein convertases modulates its inhibitory effects on lipoprotein lipase activity." in: The Journal of biological chemistry, Vol. 286, Issue 18, pp. 15747-56, 2011 (PubMed).

Liu, Afroza, Rader, Jin: "Angiopoietin-like protein 3 inhibits lipoprotein lipase activity through enhancing its cleavage by proprotein convertases." in: The Journal of biological chemistry, Vol. 285, Issue 36, pp. 27561-70, 2010 (PubMed).