驴 anti-山羊 IgG (Heavy & Light Chain) Antibody (IRDye680LT)

ABIN2169649 产品详细信息, 供应商: Log in to see
抗原
抗原表位
Heavy & Light Chain
4679
2536
1881
1066
1006
860
300
294
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
适用
山羊
2849
2739
2297
1675
1432
788
613
442
380
379
358
339
291
290
208
138
135
122
41
29
27
25
12
11
9
8
6
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
宿主

6708
4832
1794
891
609
388
91
55
52
21
8
5
5
5
2
1
1
克隆类型
多克隆
标记
IRDye680LT
2235
1872
1744
1356
767
682
519
297
264
223
222
200
156
138
128
110
79
75
69
55
51
48
46
43
40
38
34
34
32
29
27
27
27
27
27
27
27
27
22
22
19
19
19
19
15
15
15
15
15
15
14
12
12
10
10
9
9
9
9
9
9
8
8
8
8
8
8
6
6
6
6
6
5
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
应用范围
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
选项
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免疫原 Goat IgG
亚型 IgG
特异性 Goat IgG
Based on ELISA, this antibody reacts with the heavy and light chains of goat IgG and sheep IgG.
交叉反应 绵羊
交叉反应 (详细) This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with human, mouse, rabbit, rat, chicken, guinea pig, hamster, swine, and horse serum proteins, but may cross-react with immunoglobulins from other species.
产品特性 Blocking buffers and antibody diluents made with bovine serum albumin (BSA) and dry milk may contain IgG that reacts with anti-bovine IgG, anti-goat IgG, anti-horse IgG, and anti-sheep IgG antibodies. This can lead to a significant increase in background and/or reduction of secondary antibody titer for protein detection applications.
纯化方法 Immunoaffinity chromatography
研究领域 Immunology, Secondary Antibodies
应用备注 Western blot: 1:20,000 Recommended
Optimum dilutions will vary and should be determined empirically.

NOTE: When using PVDF membranes for Western blotting, SDS (final concentration of 0.1 – 0.2%) and Tween® 20 (final concentration of 0.1 – 0.2%) must be added during the detection incubation step to avoid non-specific background staining.

  • IRDye 680LT secondary antibodies are suitable for immunofluorescent microscopy and other fluorescent imaging applications when using instrumentation with appropriate excitation and detection capabilities.
  • IRDye 680LT is not intended for In-Cell WesternTM assays or in vivo applications.
  • These antibodies are not recommended when getting up and running on system. Once established near-infrared protocols are optimized with IRDye 680RD, IRDye 680LT can be used to optimize signals in the 700 channel. Dilution range 1:20,000 – 1:40,000.
    Note: optimization may be required with IRDye 680LT. Please see pack insert for specific detergents/protocol modifications needed.
  • 说明

    • IRDye 680LT secondary antibodies have been proven to provide the brightest signal for Western blot detection in the 700 nm channel and are comparable to Alexa Fluor® 680 secondary antibodies.
    • Choose IRDye 680LT secondary antibodies to get high signal and for specific uses of detection in the 700nm channel.
    • We do not recommend to start with IRDye 680LT secondaries but rather to start with one of the IRDye 680RD secondaries.

    限制 仅限研究用
    状态 Lyophilized
    溶解方式 Reconstitute with sterile, distilled water
    浓度 1.0 mg/mL
    缓冲液 PBS, pH 7.4
    储存液 Sodium azide
    注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项 Protect from light.
    储存条件 4 °C
    有引用在: Mans, Banz, Mueller, Pabst: "The angiogenesis inhibitor vasostatin is regulated by neutrophil elastase-dependent cleavage of calreticulin in AML patients." in: Blood, Vol. 120, Issue 13, pp. 2690-9, 2012 (PubMed).