Nuclear enriched lysate probed for alpha-tubulin and ELF5. The blot was probed with mouse anti-alpha-tubulin and rabbit anti-ELF5 followed by detection with IRDye® 680RD Goat anti-Mouse IgG and IRDye 800CW Goat anti-Rabbit IgG.
Dose response of EGFR phoshphorylation in A431 cells. A431 cells were treated with serial dilutions of EGF (6.3-200 ng/mL). Lysates were resolved by SDS-PAGE and transferred to Odyssey® Nitrocellulose Membrane. The blot was probed with rabbit anti-phosphoEGFR and mouse anti-ERK2 followed by detection with IRDye® 800CW Goat anti-Rabbit IgG and IRDye 680RD Goat anti-Mouse IgG.
Effect of siRNA and cytokine treatment on phosphorylation of AKT and ERK. The blot was probed with rabbit anti-ERK, mouse anti-pERK, mouse anti-AKT, and rabbit anti-pAKT primary antibodies. IRDye® 800CW goat anti-rabbit IgG and IRDye 680LT goat anti-mouse IgG were used for detection.