驴 anti-山羊 IgG (Heavy & Light Chain) Antibody (IRDye800CW)

ABIN2169607 产品详细信息, 供应商: Log in to see
抗原
抗原表位
Heavy & Light Chain
5027
2578
1931
1069
1007
857
330
299
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
1
适用
山羊
2912
2859
2405
1752
1484
796
623
442
381
380
355
340
307
293
208
148
139
114
41
29
27
25
12
11
9
8
6
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
宿主

6893
4912
1877
895
703
401
92
54
52
21
9
8
5
5
2
1
1
克隆类型
多克隆
标记
IRDye800CW
2235
1889
1740
1374
765
679
519
300
299
288
274
268
217
188
178
124
103
79
69
60
54
46
43
40
40
36
34
32
29
29
27
27
27
27
27
27
27
27
22
22
19
19
19
19
16
15
15
15
15
15
14
12
12
11
10
10
9
9
9
9
9
9
8
8
8
8
8
7
7
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
应用范围
In-Gel Western blotting (gelWB), Immunohistochemistry (IHC), Western Blotting (WB)
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品牌 In-Cell Western™,CellTag™
免疫原 Goat IgG, whole molecule
亚型 IgG
片段 H&L
特异性 Goat IgG
交叉反应 绵羊
交叉反应 (详细) The antibody also reacts with sheep IgG.
产品特性 IRDye 800CW Donkey anti-Goat IgG is a highly cross-adsorbed secondary antibody suitable for use in a variety of applications including Western blotting, In-Cell Western Assays, In-Gel Westerns, and many others. Based on immunoelectrophoresis, the antibody reacts with the heavy chains on goat IgG and with the light chains common to most goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with chicken, guinea pig, Syrian hamster, horse, human, mouse, rabbit, and rat serum proteins, but the antibody may cross-react with immunoglobulins from other species.
Blocking buffers and antibody diluents made with bovine serum albumin (BSA) and dry milk may contain IgG that reacts with anti-bovine IgG, anti-goat IgG, anti-horse IgG, and anti-sheep IgG antibodies. This can lead to a significant increase in background and/or reduction of secondary antibody titer for protein detection applications.
纯化方法 immunoaffinity chromatography
研究领域 Immunology, Secondary Antibodies
应用备注 Western blot: 1:5,000 - 1:25,000
限制 仅限研究用
状态 Lyophilized
溶解方式 Reconstitute with sterile, distilled water
浓度 1.0 mg/mL
缓冲液 PBS, pH 7.4
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Protect from light.
储存条件 4 °C
有引用在: Li, Li, Sun, Dong, Li, Zhao, Guo, Weng, Qiu: "Poly(C)-binding protein 1, a novel N(pro)-interacting protein involved in classical swine fever virus growth." in: Journal of virology, Vol. 87, Issue 4, pp. 2072-80, 2013 (PubMed).