His 标记 抗体

ABIN1981363 产品详细信息, 供应商: Log in to see
抗原
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
适用
标记
616
24
22
14
3
2
宿主
小鼠
434
184
27
23
4
4
3
1
克隆类型 (克隆位点)
单克隆 ()
标记
非结合性
59
45
36
15
14
12
11
10
10
10
10
9
8
8
8
8
7
7
6
5
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
应用范围
ELISA, Western Blotting (WB)
554
385
167
107
99
77
69
26
13
11
9
7
7
6
6
6
5
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
选项
Supplier
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原理 Monoclonal Antibody to detect His-taaged proteins
免疫原 6X-HIS-KLH
序列 HHHHHH
克隆位点 11E12
亚型 IgG1 kappa
特异性 Monoclonal Antibody detects His-taaged proteins
产品特性 Monoclonal Antibody to detect His-taaged proteins
纯化方法 Protein A chromatography from in vitro production
别名 His-Tag
物质类 Tag
背景 A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.
Synonyms: His-Tag
研究领域 Tags/Labels
应用备注 ELISA, WB
说明

Suggested positive control:
Western blot: His-Tagged protein

限制 仅限研究用
状态 Liquid
浓度 1 mg/mL
缓冲液 1 mg/mL in 0.15 M PBS
注意事项 The antibody solution should be gently mixed before use.
储存条件 -20 °C
有效期 12 months
厂商提供的图像
Western Blotting (WB) image for anti-His Tag antibody (ABIN1981363) anti-His Tag antibody
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