FAK 抗体 (pTyr925)
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- 抗原 See all FAK (PTK2) 抗体
- FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))
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抗原表位
- pTyr925
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This FAK antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF)
- 特异性
- This antibody detects endogenous levels of FAK only when phosphorylated at Tyrosine 925.
- 纯化方法
- Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- 免疫原
- The antiserum was produced against synthesized phosphopeptide derived from human FAK around the phosphorylation site of tyrosine 925 (K-V-YP-E-N).
- Top Product
- Discover our top product PTK2 Primary Antibody
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anti-PTK2 Protein tyrosine Kinase 2 (PTK2) (pTyr397) antibody
PTK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-PTK2 Protein tyrosine Kinase 2 (PTK2) (AA 700-800) antibodyPTK2 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-PTK2 Protein tyrosine Kinase 2 (PTK2) (AA 798-1041) antibodyPTK2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-PTK2 Protein tyrosine Kinase 2 (PTK2) (AA 355-490) antibodyPTK2 适用: 人 WB, ELISA, IP, PLA 宿主: 小鼠 Monoclonal 2C3 unconjugated
anti-PTK2 Protein tyrosine Kinase 2 (PTK2) (pTyr576) antibodyPTK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-PTK2 Protein tyrosine Kinase 2 (PTK2) antibodyPTK2 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-PTK2 Protein tyrosine Kinase 2 (PTK2) (AA 396-423) antibodyPTK2 适用: 人 WB, IHC (p), IF, FACS 宿主: 兔 Polyclonal RB20775 unconjugated
anti-PTK2 Protein tyrosine Kinase 2 (PTK2) (AA 1-680) antibodyPTK2 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-PTK2 Protein tyrosine Kinase 2 (PTK2) (pTyr861) antibodyPTK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-PTK2 Protein tyrosine Kinase 2 (PTK2) antibodyPTK2 适用: 人 WB, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 10H7E9 unconjugated
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- 应用备注
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Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store the antibody (in aliquots) at -20 °C.
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- 抗原
- FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))
- 别名
- FAK1 / PTK2 (PTK2 产品)
- 别名
- FADK antibody, FAK antibody, FAK1 antibody, FRNK antibody, PPP1R71 antibody, p125FAK antibody, pp125FAK antibody, PTK2 antibody, Fadk antibody, mKIAA4203 antibody, fak antibody, fak1a antibody, ptk2 antibody, ptk2a antibody, wu:fa18c04 antibody, zgc:66114 antibody, CG10023 antibody, CT28129 antibody, DFAK antibody, DFak antibody, DFak56 antibody, Dfak antibody, Dfak56 antibody, DmFAK antibody, Dmel\\CG10023 antibody, Fak56 antibody, Fak56D antibody, fak56 antibody, protein tyrosine kinase 2 antibody, protein tyrosine kinase 2 L homeolog antibody, PTK2 protein tyrosine kinase 2 antibody, protein tyrosine kinase 2ab antibody, Focal adhesion kinase antibody, PTK2 antibody, ptk2.L antibody, Ptk2 antibody, ptk2ab antibody, Fak antibody
- 背景
- Focal adhesion kinase (FAK) is a non receptor protein tyrosine kinase discovered as a substrate for Src and as a key element of integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion, however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase of the cell cycle). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly. FAK and its phosphorylation states have been implicated in cancer metastasis and tumor cell survival and adhesion-independent growth. Additionally, recent evidence indicates that elevation of FAK activity in human carcinoma cells is associated with increased invasive potential. A central role in tumor formation and progression suggests that FAK is an attractive target for therapeutic intervention.Synonyms: FADK1, FAK, Focal adhesion kinase 1, Protein-tyrosine kinase 2, pp125FAK
- 基因ID
- 5747
- NCBI登录号
- NP_722560
- UniProt
- Q05397
- 途径
- Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
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