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Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (Internal Region) 抗体

ABIN190755 产品详细信息, 供应商: Log in to see
抗原
  • p40phox
  • AI451400
  • NCF
  • P40PHOX
  • SH3PXD4
  • p40phox protein
  • neutrophil cytosolic factor 4
  • neutrophil cytosolic factor 4, 40kDa
  • p40phox
  • LOC100136241
  • Ncf4
  • NCF4
抗原表位
Internal Region
19
9
8
4
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
适用

117
58
44
7
7
5
4
4
3
3
宿主
山羊
91
19
11
克隆类型
多克隆
标记
非结合性
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
实验类型
Peptide ELISA
应用范围
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
74
40
32
26
23
5
4
3
3
2
2
1
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免疫原 Peptide with sequence C-KDFPEEDDPTN, from the internal region of the protein sequence according to NP_000622.2, NP_038202.1.
Blocking Peptide Blocking peptide for this product available: ABIN369246
特异性 This antibody is expected to recognise both reported isoforms (NP_000622.2 and NP_038202.1).
交叉反应
纯化方法 Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
别名 NCF4 / P40PHOX (NCF4 Antibody 摘要)
背景 Synonyms: NCF4, P40PHOX, neutrophil cytosolic factor 4, 40kDa, CTA-833B7.1, MGC3810, NCF, SH3PXD4, OTTHUMP00000028736, OTTHUMP00000043756, neutrophil NADPH oxidase factor 4, neutrophil cytosol factor 4, neutrophil cytosolic factor 4 (40kD)
基因ID 4689
NCBI登录号 NP_038202, NP_000622
研究领域 Autophagy
应用备注 ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 38 kDa band observed in lysates of cell line Daudi (calculated MW of 39.0 kDa according to NP_000622.2 and 38.8 kDa according to NP_038202.1). In transfected HEK293 transiently expressing P40PHOX a band of approx. 42 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 µg/mL.
IHC: In paraffin embedded Human Tonsil shows staining of the cytoplasm in some cells. Recommended concentration: 2-4 µg/mL.
限制 仅限研究用
状态 Liquid
缓冲液 Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Minimize freezing and thawing.
储存条件 -20 °C
储存方法 Aliquot and store at -20 °C.
厂商提供的图像
 image for anti-Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (Internal Region) 抗体 (ABIN190755) ABIN190755 (0.5µg/ml) staining of Daudi cell lysate (35µg protein in RIPA buffer). Pr...
背景 Rioux, Xavier, Taylor, Silverberg, Goyette, Huett, Green, Kuballa, Barmada, Datta, Shugart, Griffiths, Targan, Ippoliti, Bernard, Mei, Nicolae, Regueiro, Schumm, Steinhart, Rotter, Duerr, Cho, Daly et al.: "Genome-wide association study identifies new susceptibility loci for Crohn disease and implicates autophagy in disease pathogenesis. ..." in: Nature genetics, Vol. 39, Issue 5, pp. 596-604, 2007 (PubMed).