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Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) 抗体

ABIN1882239 产品详细信息, 供应商: Log in to see
抗原
  • erk1/2
  • mitogen-activated protein kinase
  • erk1/2
适用
人, 小鼠
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312
301
57
24
15
9
8
8
8
7
6
5
5
3
3
2
2
2
1
1
1
1
宿主
小鼠
332
75
6
克隆类型 (克隆位点)
单克隆 ()
标记
非结合性
12
11
11
9
7
7
7
6
6
6
6
6
6
6
2
2
2
1
1
1
1
1
1
应用范围
Western Blotting (WB)
291
106
81
78
61
54
39
38
14
10
6
4
3
2
2
2
2
Supplier
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免疫原 Purified His-tagged Erk1/2 protein was used to produced this monoclonal antibody.
克隆位点 784CT7-6-3
亚型 IgG2a
纯化方法 This antibody is purified through a protein G column, followed by dialysis against PBS.
别名 Erk1/2 (MAPK1/3 Antibody 摘要)
背景 Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The the MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.

Synonyms: MAPK3,ERK1, PRKM3, Mitogen-activated protein kinase 3, Mitogen-activated protein kinase 3, ERT2, Mitogen-activated protein kinase 3, Extracellular signal-regulated kinase 1, Mitogen-activated protein kinase 3, Insulin-stimulated MAP2 kinase, Mitogen-activated protein kinase 3, MAP kinase isoform p44, Mitogen-activated protein kinase 3, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 3, p44-ERK1,Mitogen-activated protein kinase 1, MAP kinase 1, MAPK 1, ERT1, Extracellular signal-regulated kinase 2, ERK-2, MAP kinase isoform p42, p42-MAPK, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2,MAPK1, ERK2,PRKM1, PRKM2
UniProt P28482, P27361
研究领域 Signaling, Inflammation
应用备注 WB: 1:1000
限制 仅限研究用
状态 Liquid
浓度 0.36 mg/mL
缓冲液 Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
储存液 Sodium azide
注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
厂商提供的图像
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) 抗体 (ABIN1882239) Erk1/2 Antibody (ABIN1882239) western blot analysis in 293,MCF-7,Jurkat,mouse NIH/3T3...
有引用在: Guo, Meng, Bai, Wei, Shi, Davis, Mao: "Prostaglandin F2? induces expression of activating transcription factor 3 (ATF3) and activates MAPK signaling in the rat corpus luteum." in: Acta histochemica, Vol. 117, Issue 2, pp. 211-8, 2015 (PubMed).

Zhao, Ye, Wu, Liu, Yang, Gao, Chen, Zhang, Yang, Li: "Sp1-CD147 positive feedback loop promotes the invasion ability of ovarian cancer." in: Oncology reports, Vol. 34, Issue 1, pp. 67-76, 2015 (PubMed).

Rönnstrand: "Signal transduction via the stem cell factor receptor/c-Kit." in: Cellular and molecular life sciences : CMLS, Vol. 61, Issue 19-20, pp. 2535-48, 2004 (PubMed).

Sano, Liu, Lane, Piletz, Lienhard: "Insulin receptor substrate 4 associates with the protein IRAS." in: The Journal of biological chemistry, Vol. 277, Issue 22, pp. 19439-47, 2002 (PubMed).

背景 Charest, Mordret, Harder, Jirik, Pelech: "Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1." in: Molecular and cellular biology, Vol. 13, Issue 8, pp. 4679-90, 1993 (PubMed).