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E-cadherin 抗体 (AA 375-631)

Name: E-cadherin 抗体 (AA 375-631)
SKU: ABIN1858334
Availability: OutOfStock
Rating: 5 (1 reviews)

CDH1 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

Immunofluorescence validation image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 375-631) antibody (ABIN1858334)

Western Blotting (WB) image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 375-631) antibody (ABIN1858334)

Western Blot; Sample: Porcine Liver lysate; Primary Ab: 5µg/ml Rabbit Anti-Porcine CDHE Antibody Second Ab: 0.

3 images

(2 references)

(1 validation)

产品编号 ABIN1858334

发货至: 中国

Datasheet as PDF

抗原 See all E-cadherin (CDH1) 抗体

E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
抗原表位
17

17

16

11

10

10

8

8

7

7

5

4

4

4

4

4

4

4

4

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 375-631
适用
226

96

83

18

16

12

9

9

9

6

4

4

4

4

1

1

1

1

1

1
Pig
宿主
192

72

7

3
兔
Compatible Secondaries
克隆类型
178

95

1
多克隆
标记
134

34

28

8

7

6

6

6

6

6

6

6

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
This E-cadherin antibody is un-conjugated
应用范围
192

87

82

64

52

50

39

34

29

27

17

16

5

5

4

4

3

3

2

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

原理

Polyclonal Antibody to E-cadherin

特异性

The antibody is a rabbit polyclonal antibody raised against E-cadherin. It has been selected for its ability to recognize E-cadherin in immunohistochemical staining and western blotting.

纯化方法

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

免疫原

Recombinant E-cadherin corresdonding to Phe375~Thr631 (Accession # C6EVT4) with N-terminal His and GST Tag

亚型

IgG

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anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) antibody
CDH1 适用: 人, 小鼠, 大鼠, 猴, 犬 WB, IF, IHC (p), IHC (fro) 宿主: 山羊 Polyclonal unconjugated

anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) antibody
CDH1 适用: 人, 小鼠, 大鼠, Pig, 猴, 犬, 小鸡, 兔, Cow, 马, 豚鼠, Avian, Cat, 驴, 山羊, Hamster, 绵羊 WB, IHC 宿主: 山羊 Polyclonal unconjugated

anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) antibody
CDH1 适用: 人 WB, IHC, IF, StM, ISt 宿主: 小鼠 Monoclonal CDH1-1525 unconjugated

anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 155-707) antibody
CDH1 适用: 人 WB, FACS, ELISA, IF 宿主: 小鼠 Monoclonal 9H2D3 unconjugated

anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) antibody
CDH1 适用: 人 WB, IHC, FACS, IF, StM, ISt 宿主: 兔 Monoclonal CDH1-2208R unconjugated Recombinant Antibody

应用备注

Western blotting: 0.5-2 μg/mL
1:160-660 Immunohistochemistry: 5-20 μg/mL
1:16-66 Immunocytochemistry: 5-20 μg/mL
1:16-66 Optimal working dilutions must be determined by end user.

说明

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

限制

仅限研究用
生效 #101345 (Immunofluorescence)

by

Sackler School of Biomedical Sciences, Tufts University School of Medicine

No.

#101345

日期

2017.06.05

抗原

E-cadherin

Lot Number

A20160427539

Method validated

Immunofluorescence

Positive Control

porcine unerupted tooth buds, dental epithelium

Negative Control

porcine unerupted tooth buds, mesenchym

Notes

Passed. The E-caherin antibody ABIN1858334 specifically labels epithelial tissues in porcine unerupted tooth buds in immunofluorescence, consistent with the expression pattern of E-cadherin.

Validation Images

A. ABIN1858334 stains the dental epithelium (DE, red) in pig tooth buds whereas the dental follicle (DF) and the pulp – labeled with the mesenchymal marker Vimentin (green) – remain unstained. B. Staining of the dental epithelial root tissue with ABIN1858334. C. Immunostaining of the DE with ABIN1858334. No staining of of dental mesenchym (DM) by ABIN1858334 or of the dental epithelium when using the secondary antibody alone (panle D). 40X, Scale Bar 50μm, 150 Resolution
Full Methods
Primary Antibody

ABIN1858334

Secondary Antibody

goat anti-rabbit AF568 conjugated antibody (ThermoFisher Scientific, A11011, lot 1778025)

Full Protocol

Harvest unerupted tooth buds from 5 month old pig jaws and wash 3x in phosphate bufferend saline (PBS) (Boston Bioproducts, BM-220).
Fix tooth buds in 10% Formalin (Fisher Scientific, 23-245-685) for 24h.
Wash 3x in PBS.
Place tooth buds through graded ethanol seris for 6h at each concentration: 50%, 70%, 80%, 90%, 100%, 100%, 100%.
Place tooth buds in 3x 2h in 100% xylene.
Place in molten paraffin 2x (TissuePrep 2 Paraffin, Fisher, T555-2) 12h.
Embed tooth buds in paraffin and cool on a 4°C cold plate for 1h.
Let set ON at RT.
Cut blocks into 6µm sections using a microtome (ThermoFisher Scientific, HM 355S).
Use a tissue floating bath to mount sections onto microscope slides.
Heat sections on a hot plate for 1h at 45°C for 1h then ON at 55°C.
Deparaffinize slide 2x 5 min in 100% xylene.
Rehydrate sections in graded ethanol series for 2min for each concentration: 100%, 95%, 70%, 50%.
Place slide in H₂O for 5min.
Transfer slide to a plastic holder filled with warmed 10mM Citric Acid Solution (made from sodium citrate monobasic; Sigma, 71497) pH6.0 and incubate in a steamer for 20min.
Cool submerged slide on benchtop for 30min.
Wash slide 3x 5min in PBS.
Create a hydrophobic barrier on slide around sections with PAP-pen (Vector Labs, H-4000, lot 5138).
Place slide in a humidified chamber and block with 5% BSA for 20min at 37°C.
Gently remove BSA by tapping slide.
Incubate sections with primary antibody
rabbit anti-pig E-cadherin antibody (antibodies-online, ABIN1858334, lot A20160408626) diluted to 1:40 in 1% BSA or
and mouse anti-pig Vimentin (Santa Cruz Biotechnology, SC-6260, lot G2814) diluted to 1:500 in 1% BSA for 1h at RT.
A no primary antibody negative control was incubated in parallel in 1% BSA.
Wash in slide 3x 5min in PBS.
Incubate sections in secondary antibodies
goat anti-rabbit AF568 conjugated antibody (ThermoFisher Scientific, A11011, lot 1778025) diluted to 1:100 in 1% BSA and
goat anti-mouse AF488 conjugated antibody (ThermoFisher Scientific, A11001, lot 1752514 ) diluted to 1:100 in 1% BSA for 1h at RT.
Wash in slide 3x 5min in PBS.
Add approximately 10μL of Hard Set Mounting Medium with DAPI (Vector Labs, H-1500, lot ZC1221) for each section and mount cover slip.

Experimental Notes
状态

Liquid

浓度

Lot specific

缓冲液

PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

储存液

Sodium azide

注意事项

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

注意事项

Avoid repeated freeze-thaw cycles.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

有效期

24 months
Smith, Zhang, Schiele, Khademhosseini, Kuo, Yelick: "Developing a biomimetic tooth bud model." in: Journal of tissue engineering and regenerative medicine, (2017) (PubMed).

Monteiro, Smith, Angstadt, Zhang, Khademhosseini, Yelick: "Dental cell sheet biomimetic tooth bud model." in: Biomaterials, Vol. 106, pp. 167-79, (2016) (PubMed).
抗原

E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

别名

E-cadherin (CDH1 产品)

别名

Arc-1 antibody, CD324 antibody, CDHE antibody, ECAD antibody, LCAM antibody, UVO antibody, E-cadherin antibody, cdh1 antibody, si:dz180o5.2 antibody, uvo antibody, lcam antibody, E-Cad antibody, XBcad antibody, l-cam antibody, xcdh1 antibody, cdhc-A antibody, xb-cad antibody, XTCAD-1 antibody, c-cadherin antibody, cadherin-1 antibody, uvomorulin antibody, XB-cadherin antibody, arc-1 antibody, cd324 antibody, cdhe antibody, ecad antibody, AA960649 antibody, E-cad antibody, Ecad antibody, L-CAM antibody, Um antibody, cadherin 1 antibody, si:busm1-180o5.2 antibody, cadherin 1, type 1, E-cadherin (epithelial) antibody, cadherin 1, type 1 S homeolog antibody, CDH1 antibody, si:busm1-180o5.2 antibody, cdh1 antibody, Cdh1 antibody, cdh1.S antibody

背景

CDHE, CD324, CDH1, Arc1, ECAD, CDH-E, E-CAD, LCAM, UVO, Cadherin 1 Type 1, Uvomorulin, E-Cadherin, Calcium-Dependent Adhesion Protein, Epithelial, Liver Cell Adhesion Molecule

途径

WNT signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation