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ERG 抗体 (C-Term)

Verified ERG 适用: 人 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN185550
发货至: 中国
  • 抗原 See all ERG 抗体
    ERG (ERG, ETS transcription factor (ERG))
    抗原表位
    • 8
    • 5
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    适用
    • 57
    • 33
    • 32
    • 6
    • 6
    • 6
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    宿主
    • 48
    • 9
    山羊
    克隆类型
    • 40
    • 17
    多克隆
    标记
    • 34
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This ERG antibody is un-conjugated
    应用范围
    • 31
    • 18
    • 14
    • 12
    • 10
    • 6
    • 6
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    原理
    ERG
    序列
    PNTRLPTSHM PSH
    特异性
    This antibody is expected to recognise all reported isoforms (NP_891548.1, NP_004440.1, NP_001129626.1, NP_001129627.1, NP_001230358.1)
    交叉反应
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-PNTRLPTSHMPSH, from the C Terminus of the protein sequence according to NP_891548.1, NP_004440.1, NP_001129626.1, NP_001129627.1, NP_001230358.1|.
    亚型
    IgG
    Top Product
    Discover our top product ERG Primary Antibody
  • 应用备注

    Western Blot: Approx 55 kDa band observed in lysates of cell line MOLT4 (calculated MW of 53.8 kDa according to NP_891548.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:16000.

    说明

    Immunofluorescence: Expression of the protein seen in the nucleus and cytoplasm of MCF7 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10u

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原
    ERG (ERG, ETS transcription factor (ERG))
    别名
    ERG (ERG 产品)
    别名
    erg-3 antibody, p55 antibody, erg antibody, erg_A antibody, zgc:103429 antibody, erg_E antibody, ERG antibody, D030036I24Rik antibody, KCNH2 antibody, ERG, ETS transcription factor antibody, v-ets avian erythroblastosis virus E26 oncogene homolog L homeolog antibody, v-ets avian erythroblastosis virus E26 oncogene homolog antibody, v-ets avian erythroblastosis virus E26 oncogene homolog S homeolog antibody, ETS transcription factor antibody, potassium voltage-gated channel subfamily H member 6 antibody, ERG antibody, Erg antibody, erg.L antibody, erg antibody, erg.S antibody, KCNH6 antibody
    背景
    ERG, v-ets erythroblastosis virus E26 oncogene like (avian), HGNC:3446, erg-3, p55, transcriptional regulator ERG (transforming protein ERG), v-ets avian erythroblastosis virus E26 oncogene related, v-ets erythroblastosis virus E26 oncogene like, v-ets er
    基因ID
    2078, 13876, 170909
    NCBI登录号
    NP_891548, NP_004440, NP_001129626, NP_001129627
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