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Androgen Receptor 抗体 (N-Term)

Verified AR 适用: 人 WB, ELISA, IHC, IF, ChIP 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN185266
发货至: 中国
  • 抗原 See all Androgen Receptor (AR) 抗体
    Androgen Receptor (AR)
    抗原表位
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    N-Term
    适用
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    宿主
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    山羊
    克隆类型
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    多克隆
    标记
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    This Androgen Receptor antibody is un-conjugated
    应用范围
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    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP)
    原理
    Androgen Receptor
    序列
    EVQLGLGRVY PRPPSC
    特异性
    This antibody is expected to recognize isoform 1 (NP_000035.2) only.
    预测反应
    Human, Mouse, Rat, Dog, Pig, Cow
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence EVQLGLGRVYPRPPSC, from the N Terminus of the protein sequence according to NP_000035.2.
    亚型
    IgG
    Top Product
    Discover our top product AR Primary Antibody
  • 应用备注

    DS WB Results: Approx 100 kDa band observed in Human Cerebellum lysates (calculated MW of 99.2 kDa according to NP_000035.2). Recommended concentration:0.1-0.5 μg/mL. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.

    DS IHC Results: In paraffin embedded Human Prostate shows nuclear staining in the secretory cells of the gland. Recommended concentration: 2-3 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:64000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the mitochondria/cytoplasm of MCF7 and U2OS cells. Recommended concentration: 10μg/ml.

    Chromatin Immunoprecipitation: A six-fold increased presence of the FKBP5 locus observed when comparing the precipitations using EB06441 with non-specific goat IgG to bring down Androgen Receptor in a chromatin lysate from DHT-stimulated HEC50 cells.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原
    Androgen Receptor (AR)
    别名
    Androgen Receptor (AR 产品)
    别名
    AIS antibody, DHTR antibody, HUMARA antibody, HYSP1 antibody, KD antibody, NR3C4 antibody, SBMA antibody, SMAX1 antibody, TFM antibody, AW320017 antibody, Tfm antibody, Andr antibody, AR antibody, androgen receptor antibody, AR antibody, Ar antibody, ar antibody
    背景
    AR, androgen receptor, dihydrotestosterone receptor, KD, AIS, TFM, DHTR, SBMA, NR3C4, SMAX1, HUMARA, HYSP1, androgen receptor, dihydrotestosterone receptor, RP11-383C12.1
    基因ID
    367
    NCBI登录号
    NP_000035
    途径
    Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding
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