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GAPDH 抗体 (C-Term)

Verified GAPDH 适用: 人, 大鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN185240
发货至: 中国
  • 抗原 See all GAPDH 抗体
    GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
    抗原表位
    • 36
    • 21
    • 13
    • 9
    • 7
    • 6
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    适用
    • 222
    • 142
    • 134
    • 75
    • 65
    • 55
    • 36
    • 28
    • 23
    • 21
    • 20
    • 19
    • 18
    • 16
    • 12
    • 12
    • 11
    • 10
    • 9
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    • 6
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    人, 大鼠
    宿主
    • 170
    • 97
    • 16
    • 10
    • 4
    山羊
    克隆类型
    • 190
    • 105
    多克隆
    标记
    • 156
    • 36
    • 23
    • 17
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GAPDH antibody is un-conjugated
    应用范围
    • 243
    • 97
    • 83
    • 69
    • 46
    • 41
    • 41
    • 35
    • 16
    • 16
    • 15
    • 15
    • 8
    • 6
    • 5
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
    原理
    GAPDH (C Terminus) Loading Control
    序列
    HQVVSSDFNS DT
    特异性
    GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.
    交叉反应
    犬, 人, 小鼠, Pig
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-HQVVSSDFNSDT, from the C Terminus of the protein sequence according to NP_002037.2.
    亚型
    IgG
    Top Product
    Discover our top product GAPDH Primary Antibody
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Pancreas. Recommended concentration: 2.5 μg/mL.

    Western Blot: Approx 36 kDa band observed in lysates of cell line HEK293, approx. 37 kDa in Rat Brain lysates and approx.35 kDa in Human Liver and Tonsil lysates, lysates of cell line HeLa and in preliminary testing of Mouse Spleen lysates (calculated MW of

    Peptide ELISA: antibody detection limit dilution 1:128000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm and vesicles of A549 and HeLa cells. Recommended concentration: 10μg/ml.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Osei Kuffour, König, Häussinger, Schulz, Münk: "ISG15 Deficiency Enhances HIV-1 Infection by Accumulating Misfolded p53." in: mBio, Vol. 10, Issue 4, (2020) (PubMed).

    George, Jacobs: "Minimal effects of spargel (PGC-1) overexpression in a Drosophila mitochondrial disease model." in: Biology open, Vol. 8, Issue 7, (2019) (PubMed).

    Hain, Krämer, Linka, Nakhaei-Rad, Ahmadian, Häussinger, Borkhardt, Münk: "IL-2 Inducible Kinase ITK is Critical for HIV-1 Infection of Jurkat T-cells." in: Scientific reports, Vol. 8, Issue 1, pp. 3217, (2019) (PubMed).

    Happold, Roth, Silginer, Florea, Lamszus, Frei, Deenen, Reifenberger, Weller: "Interferon-? induces loss of spherogenicity and overcomes therapy resistance of glioblastoma stem cells." in: Molecular cancer therapeutics, Vol. 13, Issue 4, pp. 948-61, (2014) (PubMed).

    Happold, Roth, Wick, Schmidt, Florea, Silginer, Reifenberger, Weller: "Distinct molecular mechanisms of acquired resistance to temozolomide in glioblastoma cells." in: Journal of neurochemistry, Vol. 122, Issue 2, pp. 444-55, (2013) (PubMed).

    Gramatzki, Herrmann, Happold, Becker, Gulbins, Weller, Tabatabai: "Glioma cell death induced by irradiation or alkylating agent chemotherapy is independent of the intrinsic ceramide pathway." in: PLoS ONE, Vol. 8, Issue 5, pp. e63527, (2013) (PubMed).

    Kiepe, Van Der Pas, Ciarmatori, Ständker, Schütt, Hoeflich, Hügel, Oh, Tönshoff: "Defined carboxy-terminal fragments of insulin-like growth factor (IGF) binding protein-2 exert similar mitogenic activity on cultured rat growth plate chondrocytes as IGF-I." in: Endocrinology, Vol. 149, Issue 10, pp. 4901-11, (2008) (PubMed).

    Henson, Roberts, Salimi, Vadlamudi, Hamer, Gilmore, Jarskog, Philpot: "Developmental regulation of the NMDA receptor subunits, NR3A and NR1, in human prefrontal cortex." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 18, Issue 11, pp. 2560-73, (2008) (PubMed).

    Gerstner, Bremer, Vander Heyden, Lavaute, Yin, Landry: "Brain fatty acid binding protein (Fabp7) is diurnally regulated in astrocytes and hippocampal granule cell precursors in adult rodent brain." in: PLoS ONE, Vol. 3, Issue 2, pp. e1631, (2008) (PubMed).

    Nguyen, Pace, Koller: "Mice lacking NKCC1 are protected from development of bacteremia and hypothermic sepsis secondary to bacterial pneumonia." in: The Journal of experimental medicine, Vol. 204, Issue 6, pp. 1383-93, (2007) (PubMed).

  • 抗原
    GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
    别名
    GAPDH (GAPDH 产品)
    别名
    G3PD antibody, GAPD antibody, Gapd antibody, BEST:GH12586 antibody, CG12055 antibody, Dmel\\CG12055 antibody, GA3PDH antibody, GADPH antibody, GAP antibody, GAPDH antibody, GAPDH I antibody, GAPDH-1 antibody, GAPDH1 antibody, GAPDHI antibody, Gapdh antibody, Gapdh-1 antibody, Gapdh43E antibody, gadph antibody, gapdh antibody, gapdh-1 antibody, gh12586 antibody, cb609 antibody, gapd antibody, mg:bb02e05 antibody, wu:fb33a10 antibody, wu:ft80f05 antibody, KNC-NDS6 antibody, g3pd antibody, G3PDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, Glyceraldehyde 3 phosphate dehydrogenase 1 antibody, glyceraldehyde-3-phosphate dehydrogenase S homeolog antibody, glyceraldehyde-3-phosphate dehydrogenase, type I antibody, olfactory receptor 8K3 antibody, GAPDH antibody, Gapdh antibody, Gapdh1 antibody, gapdh antibody, gapdh.S antibody, gapDH antibody, LOC100404960 antibody, LOC614985 antibody
    背景
    GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HGNC:4141, G3PD, GAPD, MGC88685, OTTHUMP00000174431, OTTHUMP00000174432, aging-associated gene 9 protein, glyceraldehyde 3-phosphate dehydrogenase
    基因ID
    2597
    NCBI登录号
    NP_002037
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