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KIF5B 抗体 (C-Term)

Verified KIF5B 适用: 人 WB, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN184774
发货至: 中国
  • 抗原 See all KIF5B 抗体
    KIF5B (Kinesin Family Member 5B (KIF5B))
    抗原表位
    • 10
    • 8
    • 7
    • 6
    • 6
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    适用
    • 48
    • 27
    • 20
    • 8
    • 7
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    宿主
    • 45
    • 7
    • 1
    山羊
    克隆类型
    • 44
    • 9
    多克隆
    标记
    • 31
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This KIF5B antibody is un-conjugated
    应用范围
    • 40
    • 21
    • 16
    • 9
    • 7
    • 6
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF)
    原理
    Kinesin 1 / UKHC
    序列
    QPVAVRGGGG KQV
    交叉反应
    Cow, 犬, 人, 小鼠, 大鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-QPVAVRGGGGKQV, from the C Terminus of the protein sequence according to NP_004512.1.
    亚型
    IgG
    Top Product
    Discover our top product KIF5B Primary Antibody
  • 应用备注

    Western Blot: Approx 110 kDa band observed in lysates of the cell lines Jurkat and HeLa (calculated MW of 110 kDa according to NP_004512.1). Recommended concentration: 0.5-1.5 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in

    Peptide ELISA: antibody detection limit dilution 1:8000.

    说明

    Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. Immunofluorescence:This antibody has been successfully used in IF on Human: Connell et al. (20

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Connell, Allison, Rodger, Pearson, Zlamalova, Reid: "ESCRT-III-associated proteins and spastin inhibit protrudin-dependent polarised membrane traffic." in: Cellular and molecular life sciences : CMLS, Vol. 77, Issue 13, pp. 2641-2658, (2020) (PubMed).

    Robinson, Evans, Briggs, Ramalho, Hume: "Inefficient recruitment of kinesin-1 to melanosomes precludes it from facilitating their transport." in: Journal of cell science, Vol. 130, Issue 12, pp. 2056-2065, (2018) (PubMed).

    Yasuda, Clatterbuck-Soper, Jackrel, Shorter, Mili: "FUS inclusions disrupt RNA localization by sequestering kinesin-1 and inhibiting microtubule detyrosination." in: The Journal of cell biology, Vol. 216, Issue 4, pp. 1015-1034, (2017) (PubMed).

    Astanina, Jacob: "KIF5C, a kinesin motor involved in apical trafficking of MDCK cells." in: Cellular and molecular life sciences : CMLS, Vol. 67, Issue 8, pp. 1331-42, (2010) (PubMed).

    Gupta, Palmer, Spence, Hudson, Stephens: "Kinesin-1 (uKHC/KIF5B) is required for bidirectional motility of ER exit sites and efficient ER-to-Golgi transport." in: Traffic (Copenhagen, Denmark), Vol. 9, Issue 11, pp. 1850-66, (2008) (PubMed).

  • 抗原
    KIF5B (Kinesin Family Member 5B (KIF5B))
    别名
    KIF5B (KIF5B 产品)
    别名
    KINH antibody, KNS antibody, KNS1 antibody, UKHC antibody, Khc antibody, AL022807 antibody, Khcs antibody, Kns1 antibody, Ukhc antibody, khc antibody, KIF5A antibody, kinesin family member 5B antibody, kinesin heavy chain antibody, kinesin-1 antibody, kinesin-1 heavy chain antibody, kinesin family member 5B S homeolog antibody, KIF5B antibody, LOC411769 antibody, cgd6_2790 antibody, kin-1 antibody, Tb927.1.1350 antibody, LACBIDRAFT_305999 antibody, Khc antibody, LOC100538629 antibody, Kif5b antibody, kif5b.S antibody
    背景
    KIF5B, UKHC, kinesin 1 (110-120kD), kinesin family member 5B, KNS, KINH, KNS1, U-KHC, kinesin heavy chain
    基因ID
    3799
    NCBI登录号
    NP_004512
    途径
    Peptide Hormone Metabolism, Ribonucleoprotein Complex Subunit Organization
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