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STAT3 抗体 (C-Term)

Verified STAT3 适用: 人, 小鼠 IHC, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN184593
发货至: 中国
  • 抗原 See all STAT3 抗体
    STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))
    抗原表位
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    C-Term
    适用
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    人, 小鼠
    宿主
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    山羊
    克隆类型
    • 195
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    多克隆
    标记
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    This STAT3 antibody is un-conjugated
    应用范围
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    Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    原理
    STAT3 (isoform 1 and 2)
    序列
    DMELTSECAT SPM
    特异性
    This antibody is expected to recognise isoforms 1 and 2 (as represented by NP_644805.1 and NP_003141.2 respectively)
    交叉反应
    Cow, 人, 小鼠, 大鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence DMELTSECATSPM, from the C Terminus of the protein sequence according to NP_003141.2, NP_644805.1.
    亚型
    IgG
    Top Product
    Discover our top product STAT3 Primary Antibody
  • 应用备注

    Immunohistochemistry: In paraffin embedded Mouse Thymus shows preferential staining of medula over cortex. Recommended concentration: 4-6 μg/mL.

    Western Blot: Preliminary testing showed a band at approx 80-85 kDa in A431 nuclear cell lysate after 2 μg/mL antibody staining (calculated MW of 80 kDa according to NP_003141.2, and NP_644805.1). Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:8000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and A431 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原
    STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))
    别名
    STAT3 (STAT3 产品)
    别名
    1110034C02Rik antibody, AW109958 antibody, Aprf antibody, APRF antibody, HIES antibody, Xstat3 antibody, aprf antibody, hies antibody, stat3 antibody, wu:fc15d02 antibody, wu:fl59g06 antibody, z-Stat3 antibody, signal transducer and activator of transcription 3 antibody, signal transduction and activation of transcription 3 antibody, signal transducer and activator of transcription 3, gene 1 L homeolog antibody, signal transducer and activator of transcription 3 (acute-phase response factor) antibody, STAT3 antibody, stat3 antibody, Stat3 antibody, stat3.1.L antibody
    背景
    STAT3, signal transducer and activator of transcription 3 (acute-phase response factor), APRF, DNA-binding protein APRF, acute-phase response factor, FLJ20882, MGC16063, HIES
    基因ID
    6774, 20848, 25125
    NCBI登录号
    NP_003141, NP_644805
    途径
    JAK/STAT Signaling, RTK signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor
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