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V5 Epitope 标记 抗体

ABIN1842466 产品详细信息, 供应商: Log in to see
抗原
适用
标记
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克隆类型
多克隆
标记
非结合性
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应用范围
ELISA, Western Blotting (WB)
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Supplier
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免疫原 V5 epitope tag peptide GKPIPNPLLGLDST conjugated to KLH
序列 GKPIPNPLLG LDST
亚型 IgG
特异性 Rabbit Anti-V5-tag Polyclonal Antibody specifically recognizes V5 tagged fusion proteins.
产品特性 V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.
纯化方法 Immunoaffinity chromatography
物质类 Tag
背景 The V5 epitope tag is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).
研究领域 Tags/Labels
应用备注 Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors,
Including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0. 05-0. 2 µg/mL
Western blot: 0. 1-1. 0 µg/mL
Western blot using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit L00204, C is recommended. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane. Other applications: user-optimized
限制 仅限研究用
状态 Lyophilized
溶解方式 Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.
浓度 0.5 mg/mL
缓冲液 lyophilized with PBS, pH 7.4, containing 0.02 % sodium azide.
储存液 Sodium azide
注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项 Avoid repeated freezing and thawing.
储存条件 -20 °C
储存方法 The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
有引用在: Afrazi, Sodhi, Good, Jia, Siggers, Yazji, Ma, Neal, Prindle, Grant, Branca, Ozolek, Chang, Hackam: "Intracellular heat shock protein-70 negatively regulates TLR4 signaling in the newborn intestinal epithelium." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 9, pp. 4543-57, 2012 (PubMed).