This antibody clone 2A11 recognises beta-Glucan Receptor, also known as Dectin-1. This antibody inhibits the binding of zymosan to macrophages via the beta-glucan receptor. The low endotoxin version (SM2025LE) is recommended for functional studies.
纯化方法
Affinity Chromatography on Protein G
内毒素水平
Low Endotoxin
免疫原
Dectin-1 transfected NIH3T3 cells and recombinant soluble Dectin-1. Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line.
Functional Assays. Immunoprecipitation. Immunohistochemistry on frozen sections: The epitope recognised by 2A11 is sensitive to H202 treatment, therefore quenching with 0.3 % H202 should be performed after incubation with the primary antibody. Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS, pH 7.4, None, None
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
抗原
CLEC7A
(C-Type Lectin Domain Family 7, Member A (CLEC7A))
Dectin-1 is predominantly expressed by cells of the monocyte/macrophage and neutrophil lineages, but also at lower levels by dendritic cells and a subpopulation of T cells. As a major leucocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of beta-glucans and in the host response to fungal pathogens. Recent work has shown that Dectin-1 may stimulate reactive oxygen production in macropahges via the protein tyrosine kinase known as Syk.Synonyms: BGR, Beta-glucan receptor, C-type lectin domain family 7 member A, C-type lectin superfamily member 12, CLECSF12, DC-associated C-type lectin 1, DECTIN1, Dendritic cell-associated C-type lectin 1