This Macrophage Mannose Receptor 1 antibody is conjugated to Biotin
应用范围
Flow Cytometry (FACS)
特异性
This antibody recognises the mannose receptor, a 175 kDa type 1 membrane protein that is also known as CD206. Clone MR5D3 has been reported to be non-inhibitory for the binding of the mannose receptor to carbohydrate ligands.
纯化方法
Affinity chromatography on Protein G
免疫原
Chimaeric CRD4-7-Fc protein. Spleen cells from immunised Fischer rats were fused with cells of the Y3 myeloma cell line.
Flow Cytometry: Neat - 1/10, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.1 mg/mL
缓冲液
PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Terminel, Bassil, Rau, Trevino, Ruiz, Alaniz, Hook: "Morphine-induced changes in the function of microglia and macrophages after acute spinal cord injury." in: BMC neuroscience, Vol. 23, Issue 1, pp. 58, (2022) (PubMed).
抗原
Macrophage Mannose Receptor 1 (MRC1)
(Mannose Receptor, C Type 1 (MRC1))
CD206 is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia.Synonyms: C-type lectin domain family 13 member D, C-type lectin domain family 13 member D-like, CLEC13D, CLEC13DL, MRC1L1, Macrophage mannose receptor, Macrophage mannose receptor 1-like protein 1