This antibody binds to mast cell chymase, it may be used to detect mast cells in routinely fixed tissues, and has been used to study the distribution of mast cells in skin, synovium, lung and heart. Binding of this antibody to chymase is not reduced in the presence of heparin, suggesting that the antibody does not bind to the heparin-binding domain of chymase.
纯化方法
Affinity Chromatography on Protein G.
免疫原
Purified human skin chymase. Spleen cells from immunised BALB/c mice were fused with cells of the P3/NSI/I.Ag4.1 mouse myeloma cell line.
ELISA. Western Blot. Immunohistochemistry on frozen sections: 1/500 - 1/1000, This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended Positive Control: Skin Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS, 0.09 % Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Mast Cell Chymase is an important marker of mast cells and also an important mediator of inflammation.Synonyms: Alpha-chymase, CMA1, CYH, CYM, Mast cell protease I