SERPINB9 抗体
-
- 抗原 See all SERPINB9 抗体
- SERPINB9 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 9 (SERPINB9))
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This SERPINB9 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)
- 特异性
- This antibody is specific for SERPINB9/PI-9 (Proteinase Inhibitor 9). Does not react with Mouse and Pig.
- 产品特性
- Synonyms: PI9, Serpin B9, Cytoplasmic antiproteinase 3, CAP-3, CAP3, Proteinase inhibitor 9
- 纯化方法
- Affinity Chromatography on Protein G
- 免疫原
- Recombinant PI-9 produced in P. pastoris. Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS-1myeloma cell line. Genename: PI-9
- 克隆位点
- 7D8
- 亚型
- IgG1
- Top Product
- Discover our top product SERPINB9 Primary Antibody
-
anti-serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 9 (SERPINB9) (AA 1-376) antibody
SERPINB9 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 9 (SERPINB9) (AA 1-376) antibodySERPINB9 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 9 (SERPINB9) antibodySERPINB9 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 9 (SERPINB9) (AA 279-375) antibodySERPINB9 适用: 人 ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1F5 unconjugated
anti-serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 9 (SERPINB9) (Internal Region) antibodySERPINB9 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 9 (SERPINB9) (Internal Region) antibodySERPINB9 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 9 (SERPINB9) (AA 1-376) antibodySERPINB9 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 9 (SERPINB9) (AA 279-375) antibodySERPINB9 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 9 (SERPINB9) (Internal Region) antibodySERPINB9 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 9 (SERPINB9) (AA 122-150) antibodySERPINB9 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
Western Blot: This antibody detects a band of approx. 42 kda under Reducing ConditionsFlow Cytometry: Use 10 μL of 1/10-1/100 diluted antibody to label 1x10^6 cells in 100 μL. Membrane permeabilisation is required for this application (The use of Leucoperm isrecommended for this purpose). Immunohistochemistry on Paraffin Sections: 1/50. This product requires antigen retrieval using heat treatment prior to staining of paraffinsections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Tonsil. Also Suitable for use in Indirect Immunofluorescence. (See Ref.2 for more details)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
-
- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, 0.05 % Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
-
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - 有效期
- 12 months
-
- 抗原
- SERPINB9 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 9 (SERPINB9))
- 别名
- SERPINB9 (SERPINB9 产品)
- 别名
- SERPINB9 antibody, BB283241 antibody, CAP-3 antibody, CAP3 antibody, PI-9 antibody, PI9 antibody, Spi6 antibody, ovalbumin antibody, serpin family B member 9 antibody, serpin peptidase inhibitor, clade B (ovalbumin), member 9 antibody, serine (or cysteine) peptidase inhibitor, clade B, member 9 antibody, Serpinb9 antibody, SERPINB9 antibody
- 背景
- PI-9 is a 42 kDa intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells. Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virusinfected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves.Synonyms: CAP-3, CAP3, Cytoplasmic antiproteinase 3, PI-9, PI9, Proteinase inhibitor 9, Serpin B9
- 基因ID
- 5272
- UniProt
- P50453
-
