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Retinoblastoma 1 抗体 (RB1)

Details for Product anti-RB1 Antibody No. ABIN180306, 供应商: Log in to see
抗原
  • RB1
  • OSRC
  • RB
  • p105-Rb
  • pRb
  • pp110
  • Rb
  • Rb-1
  • zgc:154147
  • P105-RB
  • PP110
  • RB11
  • retinoblastoma 1 (including osteosarcoma)
  • retinoblastoma 1
  • Retinoblastoma-associated protein
  • RB1
  • rb
  • Rb1
  • rb1
Alternatives
anti-人 Retinoblastoma 1 抗体 for BioImaging
适用

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宿主
小鼠
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克隆类型 (克隆位点)
单克隆 ()
标记
This Retinoblastoma 1 antibody is un-conjugated
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应用范围
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
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Supplier
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免疫原 Recombinant human retinoblastoma gene protein.
克隆位点 1F8
亚型 IgG1
特异性 This antibody recognises the Human retinoblastoma (Rb) gene product and specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It reacts with hyperphosphorylated and un (under) phosphorylated form of Rb protein. It shows no cross reaction with p107 and p130.
Cellular Localization: Nuclear. Does not react with Mouse and Rat.
产品特性 Synonyms: Retinoblastoma 1, Rb, p105-Rb, pRb, pp110, OSRC
纯化方法 Purified from the ascites fluid by Protein G Chromatography
别名 Retinoblastoma-Associated Protein / RB1 (RB1 Antibody 摘要)
背景 Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.Synonyms: OSRC, Rb, Retinoblastoma 1, p105-Rb, pRb, pp110
分子量 105 kDa
基因ID 5925
UniProt P06400
途径 Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy
应用备注 Flow Cytometry, Immunofluorescence, Immunoprecipitation (Native and denatured): useProtein G and Ab at 2 g/mg protein lysate, Western Blotting (Ab 1-2g/mL for 2hrs at RT),Immunohistology (Formalin/paraffin: Ab 1-2g/mL for 30 min at RT) Staining offormalin-fixed tissues REQUIRES boiling tissue sections in 1 mM EDTA, pH 8.0 for 10-20 min. followed by cooling at RT for 20 min. EDTA is better than citrate for epitopeunmasking. Recommended positive control: Ls174T, HT29, HUVEC cells. Normal colon andcarcinoma. Breast carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
浓度 0.2 mg/mL
缓冲液 10 mM PBS, pH 7.4, 0.09 % Sodium Azide, 0.2 % BSA
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C/-20 °C
储存方法 Store the antibody at 4-8 °C for one month or at -20 °C for longer. Avoid repeated freezingand thawing.
Shelf life: one year from despatch.
有效期 12 months
厂商提供的图像
Immunohistochemistry (IHC) image for anti-Retinoblastoma 1 抗体 (RB1) (ABIN180306) anti-Retinoblastoma 1 (RB1) antibody
背景 Bártek, Vojt?sek, Grand, Gallimore, Lane: "Cellular localization and T antigen binding of the retinoblastoma protein." in: Oncogene, Vol. 7, Issue 1, pp. 101-8, 1992 (PubMed).

Grand, Byrd, Grabham, Gregory, Huen, Merrick, Young, Gallimore: "The expression of the retinoblastoma gene product Rb1 in primary and adenovirus-transformed human cells." in: Oncogene, Vol. 4, Issue 11, pp. 1291-8, 1989 (PubMed).