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Interleukin 8 抗体 (IL8)

Details for Product anti-IL8 Antibody No. ABIN1724413, 供应商: Log in to see
抗原
  • IL8
  • K60
  • interleukin-8
  • il8
  • si:dkey-151b16.2
  • cxcli2
  • Il8
  • Scyb15
  • lungkine
  • weche
  • CXCL8
  • GCP-1
  • GCP1
  • LECT
  • LUCT
  • LYNAP
  • MDNCF
  • MONAP
  • NAF
  • NAP-1
  • NAP1
  • IL-8
  • AMCF-I
  • EMF-1
  • interleukin 8-like 1
  • interleukin 8
  • chemokine (C-X-C motif) ligand 1-like
  • chemokine (C-X-C motif) ligand 15
  • interleukin 8-like 2
  • interleukin-8-like
  • IL8L1
  • il8
  • IL8
  • LOC422654
  • Cxcl15
  • Il8
  • IL8L2
  • IL*08-02
Alternatives
anti-人 Interleukin 8 抗体 for Blocking Antibody
适用

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宿主
小鼠
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克隆类型
单克隆
标记
This Interleukin 8 antibody is un-conjugated
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应用范围
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
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Supplier
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Available images

'独立验证'标志
  • 成功验证 (Immunohistochemistry)
  • 通过  HIK Hannover
  • 编号 #100789
  • 日期 2017.05.31
  •  
抗原 IL8
Method validated Immunohistochemistry
Positive Control Formalin fixed and plastic embedded human liver, testicle, and tonsil tissue sections
Negative Control No primary antibody
Notes Passed. The IL8 antibody ABIN1724413 specifically labels the targeted antigen in human liver, testicle, and tonsil sections in IHC. No signal was detected in tonsil negative control tissue sections and the no primary antibody negative controls.
Primary Antibody ABIN1724413
Secondary Antibody Histofine Simple Stain MAX PO (M) Anti-Mouse (Medac Diagnostika, 414132F, lot no H1608)
实验流程
  • Disect tissue and fix in 4% buffered formalin for >24h at RT.
  • Plastification in Technovit 9100 plastic embedding system (Heraeus Kulzer) according to manufactuer’s recommencations.
  • Sectioning into 5µm sections on a rotary microtome (Reichert Jung, 1140/Autocut).
  • Deplastify and rehydrate tissue through graded xylene and graded alcohol series:
    • xylene for 20min.
    • MEA (2-Methoxyethyl)acetat zur Synthese (Merck, 8.06061.1000) 2x 20min.
    • 100% ethanol for 1min.
    • 95% ethanol for 1min.
    • 70% ethanol for 1min.
    • H2O for 2min.
    • distilled H2O 2min.
  • Heat induced epitope retrieval in high pH Trilogy buffer (Medac Diagnostika, 920P-07, lot 1622912A) ON at 60°C in an oven (Memmert, UNB 200).
  • Quench sections in in ice cold distilled H2O 2min.
  • Adjust sections with wash buffer (Medac Diagnostika, B1-30A, lot no 161102001) 5min at RT.
  • Incubate sections with primary Interleukin 8 antibody (antibodies-online, ABIN1724413) diluted 1:400 in dilution buffer (Medac Diagnostika, B1-31C, lot no 161205001) for 30min at RT. Includeno primaryantibody negative controls.
  • Rinse sections 3x 5min with wash buffer. Keep negative controls in a separate container.
  • Block endogenous peroxidase with Peroxide Block RTU (Medac Diagnostika, 925B-09, lot no 161103001) for 10min at RT.
  • Rinse sections 3x 5min with wash buffer.
  • Incubate sections with Histofine Simple Stain MAX PO (M) Anti-Mouse (Medac Diagnostika, 414132F, lot no H1608) for 30min at RT.
  • Rinse sections 3x 5min with wash buffer.
  • Incubate sections with Bright DAB (Medac Diagnostika, BS04-110, lot no 160523) for 10min at RT.
  • Rinse sections in distilled H2O.
  • Counterstain with Mayers Haematoxylin (Leica, 3801582E, lot no3801582E) 5min at RT.
  • Dehydrate in fresh ethanol:
    • 95% ethanol twice for 2min.
    • 99.5% ethanol twice for 2min.
    • Xylene twice for 2min.
  • Mount sections with Celltexx mounting medium (Engelbrecht, 19360, lot no 1716047).
  • Dry sections in a ventilated place.
  • Image acquisition on a Leica Microsystems DM 1000 light microscope with a DFC 320 digital camera. Magnification tonsil sections 40x, liver sections 10x and 20x, testicle sections 10x.
Experimental Notes Immunostaining pattern of human liver, testicle, and tonsil with ABIN1724413 is consistent with published data.
Validation Images
Immunohistochemistry validation image for anti-Interleukin 8 (IL8) antibody (ABIN1724413) Immunostaining of IL8 in human liver liver (A), testicle (C), and tonsil sections (D)...
免疫原 Recombinant protein corresponding to full length IL8
亚型 IgG1
特异性 Reacts with human IL-8
纯化方法 Purified
别名 IL-8 (IL8 Antibody 摘要)
背景 IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
基因ID 3576
研究领域 Cytokines, Immunology, Virology, Inflammation, Cancer
途径 Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy
应用备注

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
IHC-P (5 μg/mL) WB (1:1000)

限制 仅限研究用
状态 Liquid
浓度 1 mg/mL
缓冲液 Citrate-Tris-HCl buffer, pH 7.0, 0.02 % Proclin 300
储存液 ProClin
注意事项 This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C/-20 °C
储存方法 Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
厂商提供的图像
 image for anti-Interleukin 8 抗体 (IL8) (ABIN1724413) anti-Interleukin 8 (IL8) antibody