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V5 Epitope 标记 抗体 (HRP)

ABIN1573947 产品详细信息, 供应商: Log in to see
抗原
适用
标记
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克隆类型
多克隆
标记
HRP
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应用范围
ELISA, Western Blotting (WB)
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Supplier
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免疫原 V5 epitope tag peptide GKPIPNPLLGLDST conjugated - KLH
序列 GKPIPNPLLG LDST
亚型 IgG
特异性 Rabbit Anti-V5-tag [HRP] Polyclonal Antibody specifically recognizes C-terminal, N-terminal, and internal V5 tagged fusion proteins.
纯化方法 Immunoaffinity chromatography
别名 V5-Tag
物质类 Tag
背景 Rabbit Anti-V5-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. Rabbit Anti-V5-tag [HRP] polyclonal antibody is suitable for detecting fusion proteins that contain V5-tags.
研究领域 Tags/Labels
应用备注 Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mL
Other applications: user-optimized
限制 仅限研究用
状态 Liquid
缓冲液 PBS, pH 7.4, containing 1 % BSA and 0.01 % thimerosal
储存液 Thimerosal (Merthiolate)
注意事项 This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C/-20 °C
储存方法 The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
厂商提供的图像
Western Blotting (WB) image for anti-V5 Epitope 标记 抗体 (HRP) (ABIN1573947) Lane 1-2: V5-tag fusion protein in Hela cell lysate (~52 kD) Lane 3: Negative Hela ce...
有引用在: Dahl, Corydon, Ränkel, Nielsen, Füchtbauer, Knudsen: "An eEF1A1 truncation encoded by PTI-1 exerts its oncogenic effect inside the nucleus." in: Cancer cell international, Vol. 14, Issue 1, pp. 17, 2014 (PubMed).

Tahir, Yang, Goltsov, Song, Ren, Wang, Chang, Thompson: "Caveolin-1-LRP6 signaling module stimulates aerobic glycolysis in prostate cancer." in: Cancer research, Vol. 73, Issue 6, pp. 1900-11, 2013 (PubMed).

Zhang, Li, Jaiswal, Bayat, Xiong, Sandoval, Charng, David, Haueter, Yamamoto, Graham, Bellen: "The C8ORF38 homologue Sicily is a cytosolic chaperone for a mitochondrial complex I subunit." in: The Journal of cell biology, Vol. 200, Issue 6, pp. 807-20, 2013 (PubMed).