(3 references)-
- 抗原 See all Podoplanin (PDPN) 抗体
- Podoplanin (PDPN)
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Podoplanin antibody is conjugated to Biotin
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应用范围
- Flow Cytometry (FACS)
- 特异性
- This antibody reacts with Podoplanin (gp36). The unconjugated monoclonal antibody will detect native Human Podoplanin in Western Blot and on the surface of Human LEC.
- 交叉反应 （详细）
- Species reactivity (tested):Human.
- 纯化方法
- Affinity Chromatography on Protein G
- 免疫原
- Podoplanin-expressing MDCK cells.
- 克隆位点
- 18H5
- 亚型
- IgG1
- Top Product
- Discover our top product PDPN Primary Antibody
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anti-Podoplanin (PDPN) (AA 91-166) antibody
PDPN 适用: 小鼠, 大鼠 WB, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Podoplanin (PDPN) (AA 23-143) antibodyPDPN 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Podoplanin (PDPN) (AA 24-126) antibodyPDPN 适用: 人 ELISA, IF, Coat, IHC (f) 宿主: 小鼠 Monoclonal PDPN-1433 unconjugated
anti-Podoplanin (PDPN) antibodyPDPN 适用: 小鼠 WB, FACS, IP, IHC (p) 宿主: 大鼠 Monoclonal 8F11 unconjugated
anti-Podoplanin (PDPN) (N-Term) antibodyPDPN 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Podoplanin (PDPN) antibodyPDPN 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Podoplanin (PDPN) antibodyPDPN 适用: 小鼠 WB, IP, IHC (fro) 宿主: Golden Syrian Hamster Monoclonal 811 unconjugated
anti-Podoplanin (PDPN) (AA 1-100) antibodyPDPN 适用: Synthetic ELISA, IF, Coat, IHC (f) 宿主: 兔 Monoclonal unconjugated Recombinant Antibody
anti-Podoplanin (PDPN) (AA 1-206) antibodyPDPN 适用: 人 ELISA, FACS, IF, ICC 宿主: 小鼠 Monoclonal 5E2 unconjugated
anti-Podoplanin (PDPN) (AA 97-146) antibodyPDPN 适用: 人, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Flow cytometry: use at 2-5 μg/mL together with the appropriate secondary reagents.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL. Centrifuge vial prior to opening.
- 缓冲液
- PBS, 0.02 % Sodium Azide, BSA (50x)
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
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: "Multipotent mesenchymal stem cells acquire a lymphendothelial phenotype and enhance lymphatic regeneration in vivo." in: Circulation, Vol. 119, Issue 2, pp. 281-9, (2009) (PubMed).
: "Wolbachia lipoprotein stimulates innate and adaptive immunity through Toll-like receptors 2 and 6 to induce disease manifestations of filariasis." in: The Journal of biological chemistry, Vol. 284, Issue 33, pp. 22364-78, (2009) (PubMed).
: "Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1." in: Blood, Vol. 110, Issue 13, pp. 4223-33, (2007) (PubMed).
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: "Multipotent mesenchymal stem cells acquire a lymphendothelial phenotype and enhance lymphatic regeneration in vivo." in: Circulation, Vol. 119, Issue 2, pp. 281-9, (2009) (PubMed).
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- 抗原
- Podoplanin (PDPN)
- 别名
- Podoplanin (PDPN 产品)
- 别名
- PDPN antibody, AGGRUS antibody, GP36 antibody, GP40 antibody, Gp38 antibody, HT1A-1 antibody, OTS8 antibody, PA2.26 antibody, T1A antibody, T1A-2 antibody, OTS-8 antibody, RANDAM-2 antibody, T1-alpha antibody, T1a antibody, T1alpha antibody, E11 antibody, RTI40 antibody, podoplanin antibody, PDPN antibody, Pdpn antibody
- 背景
- Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential Olinked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47 % aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for Ctype lectin-like receptor 2 (CLEC2). Their association is dependent on sialic acid on Oglycans of Podoplanin. Through its association with CLEC2, Podoplanin-induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.Synonyms: Aggrus, GP36, Glycoprotein 36, PA2.26 antigen, PDPN, PSEC0003, PSEC0025, T1-alpha
- 基因ID
- 10630
- NCBI登录号
- NP_001006625
- UniProt
- Q86YL7
- 途径
- Dicarboxylic Acid Transport
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