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EIF4E 抗体 (AA 121-217)

EIF4E 适用: 人, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN1386772
发货至: 中国
  • 抗原 See all EIF4E 抗体
    EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))
    抗原表位
    • 38
    • 14
    • 8
    • 7
    • 7
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 121-217
    适用
    • 125
    • 61
    • 50
    • 19
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    人, 大鼠
    宿主
    • 107
    • 18
    克隆类型
    • 98
    • 27
    多克隆
    标记
    • 50
    • 11
    • 10
    • 7
    • 7
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    This EIF4E antibody is un-conjugated
    应用范围
    • 96
    • 54
    • 41
    • 27
    • 26
    • 25
    • 18
    • 16
    • 15
    • 15
    • 6
    • 6
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
    交叉反应
    人, 大鼠
    预测反应
    Mouse
    纯化方法
    Purified by Protein A.
    免疫原
    KLH conjugated synthetic peptide derived from human eIF4E
    亚型
    IgG
  • 应用备注
    WB 1:300-5000
    ELISA 1:500-1000
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    ICC 1:100-500
    限制
    仅限研究用
  • 生效 #029640 (Immunohistochemistry)
    '独立验证'标志
    by
    Immunohistochemistry Core, NYU Langone
    No.
    #029640
    日期
    2014.03.14
    抗原
    Lot Number
    140106
    Method validated
    Immunohistochemistry
    Positive Control
    Human pancreas
    Negative Control
    Human colon
    Notes
    Clear strong signal was detected in positive control sample. Note a minor amount of expected background staining in negative control sample.
    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    • Antigen: Eukaryotic Translation Initiation Factor 4E (EIF4E)
    • Catalog number: ABIN1386772
    • Lot number: 140106
    Secondary Antibody
    • Antibody: Biotinylated goat anti-rabbit/anti-mouse (Kit)
    • Lot number: D07640BA
    Full Protocol
    • Immunohistochemistry was performed on a Ventana NEXes automated platform; instrument manufacturer specific reagents are italicized.
    • 1. Slides were preheated in convection oven at 60°C for 30 min
    • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
    • 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
    • 4. NEXes instrument procedure, iView DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
    • 5. Slides rinsed with *reaction buffer* x3
    • 6. *iView Inhibitor (H2O2)* applied and incubated for 4 min
    • 7. Slides rinsed with *reaction buffer*
    • 8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliter applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
    • 9. Slides rinsed with *reaction buffer* x3
    • 10. *iView Biotinylated IgG* applied and incubated for 8 min
    • 11. Slides rinsed with *reaction buffer*
    • 14. *iView Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
    • 15. Slides rinsed with *reaction buffer*
    • 16. *iView DAB/H2O2* applied and incubated for 8 min
    • 17. Slides rinsed with *reaction buffer*
    • 18. *iView Copper* applied and incubated for 4 min
    • 19. Slides rinsed with *reaction buffer*
    • 20. Slides washed in Dawn Detergent/tap water
    • 21. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 sec - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
    • 22. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
    • 23. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
    Experimental Notes
    • Deviations from protocol/procedure supplied by manufacturer (attached).
    • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
    • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
    • Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
    • Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
    • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
    • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
    • Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
    • Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
  • 状态
    Liquid
    浓度
    1 μg/μL
    缓冲液
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    储存液
    ProClin
    注意事项
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    有效期
    12 months
  • 抗原
    EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))
    别名
    eIF4E (EIF4E 产品)
    别名
    AUTS19 antibody, CBP antibody, EIF4E1 antibody, EIF4EL1 antibody, EIF4F antibody, eif4e-1 antibody, zgc:86680 antibody, EG668879 antibody, Eif4e-ps antibody, If4e antibody, eIF-4E antibody, 0260/09 antibody, 0587/11 antibody, 0589/11 antibody, 0919/12 antibody, 1004/13 antibody, 2 antibody, 7238 antibody, CG4035 antibody, CT13384 antibody, CT39424 antibody, CT39426 antibody, D-eIF4E antibody, Dmel\\CG4035 antibody, Eif4E antibody, Eif4e antibody, IF4E antibody, dEif4e antibody, deIF-4E antibody, deIF4E antibody, eIF 4E antibody, eIF-4E1 antibody, eIF-4E2 antibody, eIF-4EII antibody, eIF-4e antibody, eIF4E antibody, eIF4E-1 antibody, eIF4E-1/2 antibody, eIF4E-2 antibody, eIF4E1 antibody, eIF4EI antibody, eIF4e antibody, eif-4E antibody, eif-4e antibody, eif4e antibody, l(3)07238 antibody, l(3)67Af antibody, l(3)S025007 antibody, l(3)S026009 antibody, l(3)S058711 antibody, l(3)S091912 antibody, cbp antibody, eif4f antibody, eif4e1 antibody, eif4el1 antibody, EIF-4E antibody, zgc:101581 antibody, eukaryotic translation initiation factor 4E antibody, eukaryotic translation initiation factor 4ea antibody, Eukaryotic initiation factor 4E antibody, eucaryotic initiation factor 6 antibody, eukaryotic translation initiation factor 4E L homeolog antibody, eukaryotic translation initiation factor 4E family member 1B S homeolog antibody, translation initiation factor eIF4E antibody, eukaryotic translation initiation factor 4eb antibody, EIF4E antibody, eif4ea antibody, Eif4e antibody, eIF-4E antibody, eif6 antibody, eif4e antibody, eif4e.L antibody, eif4e1b.S antibody, LOC100533326 antibody, eif4eb antibody
    背景

    Synonyms: CBP, EIF4F, AUTS19, EIF4E1, EIF4EL1, Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein

    Background: Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.

    基因ID
    1977
    UniProt
    P06730
    途径
    BCR Signaling
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