Phosphotyrosine 抗体 (Alkaline Phosphatase (AP))
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- 抗原 See all Phosphotyrosine products
- Phosphotyrosine
- 适用
- 请咨询
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Phosphotyrosine antibody is conjugated to Alkaline Phosphatase (AP)
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- 产品特性
- The antibody detects: Phosphorylated tyrosine and phenylphosphate
- 纯化方法
- Purified
- 克隆位点
- PY20
- 亚型
- IgG2b
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anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Phosphotyrosine antibodyWB, IP, IF, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal E10 unconjugated
anti-Phosphotyrosine antibodyWB, IP, IHC, FACS, ELISA, ICC 宿主: 兔 Monoclonal RM111 unconjugated
anti-Phosphotyrosine antibody适用: Various Species WB, FACS, ICC 宿主: 小鼠 Monoclonal P-Tyr-01 unconjugated
anti-Phosphotyrosine antibody (FITC)WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal FITC
anti-Phosphotyrosine antibody (Atto 488)WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal Atto 488
anti-Phosphotyrosine antibody (Atto 594)WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal Atto 594
anti-Phosphotyrosine antibody (Atto 390)WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal Atto 390
anti-Phosphotyrosine antibodyWB, ELISA 宿主: 小鼠 Monoclonal unconjugated
anti-Phosphotyrosine antibody适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
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- 应用备注
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Working Dilution:
ELISA: 1:500-1:1,000
Representative data are included in this product insert.
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. - 样本量
- 1 mL
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Product is supplied as 1.0 mL of stock solution in 50 mM Tris / 0 mM MgCl2 / 50 % Glycerol, pH 8.0, containing 0.1 % NaN3 as preservative.
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
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Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed. - 储存条件
- 4 °C
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- 抗原
- Phosphotyrosine
- Abstract
- Phosphotyrosine 产品
- 物质类
- Amino Acid
- 背景
- Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g. via growth factors) during normal growth and development, and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation are cell surface glycoproteins. Antibody to phosphotyrosine enables the detection, isolation and characterization of proteins containing phosphotyrosine. PY20 also prevents internalization of activated receptors, e.g. EGFr, when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10-6 to 10-7 M. PY20 binding to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by phosphoserine, phosphothreonine or free phosphate.
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