-
- 抗原 See all Phosphotyrosine products
- Phosphotyrosine
- 适用
- 请咨询
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This Phosphotyrosine antibody is un-conjugated
-
应用范围
- ELISA
- 特异性
- Tyrosine-phosphorylated proteins
- 产品特性
- Mouse Anti-Phosphotyrosine-UNLB
- 纯化方法
- Purified
- 免疫原
- Phosphotyrosine conjugated to carrier proteins
- 克隆位点
- PY20
- 亚型
- IgG2b
-
anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Phosphotyrosine antibodyWB, IP, IF, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal E10 unconjugated
anti-Phosphotyrosine antibodyWB, IP, IHC, FACS, ELISA, ICC 宿主: 兔 Monoclonal RM111 unconjugated
anti-Phosphotyrosine antibody适用: Various Species WB, FACS, ICC 宿主: 小鼠 Monoclonal P-Tyr-01 unconjugated
anti-Phosphotyrosine antibody (FITC)WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal FITC
anti-Phosphotyrosine antibody (Atto 488)WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal Atto 488
anti-Phosphotyrosine antibody (Atto 594)WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal Atto 594
anti-Phosphotyrosine antibody (Atto 390)WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal Atto 390
anti-Phosphotyrosine antibodyWB, ELISA 宿主: 小鼠 Monoclonal unconjugated
anti-Phosphotyrosine antibody适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
-
- 应用备注
-
- Applications: ELISA - Quality tested , FLISA - Quality tested WB - Reported in literature , IP - Reported in literature , IHC-PS - Reported in literature , IHC-WM - Reported in literature , ICC - Reported in literature , FC - Reported in literature , Microarray - Reported in literature , Purification - Reported in literature
- Working Dilutions: ELISA AP conjugate 1:1,000 - 1:2,000 HRP conjugate 1:1,000 - 1:4,000 BIOT conjugate 1:5,000 - 1:10,000 FLISA FITC conjugate 1:200 - 1:400 PE conjugate ≤ 1 g/mL
- 样本量
- 1 mL
- 限制
- 仅限研究用
-
- 浓度
- 0.5 mg/mL
- 缓冲液
- 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added
- 储存液
- Without preservative
- 注意事项
- Each reagent is stable for the period shown on the bottle label if stored as directed.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C
-
- 抗原
- Phosphotyrosine
- Abstract
- Phosphotyrosine 产品
- 物质类
- Amino Acid
- 背景
- Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g. via growth factors) during normal growth and development, and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation are cell surface glycoproteins. Antibody to phosphotyrosine enables the detection, isolation and characterization of proteins containing phosphotyrosine. PY20 also prevents internalization of activated receptors, e.g. EGFr, when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10-6 to 10-7 M. PY20 binding to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by phosphoserine, phosphothreonine or free phosphate.
-
You are here:
